+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Protein distribution in the human perilymph. A comparative study between perilymph (post mortem), CSF and blood serum



Protein distribution in the human perilymph. A comparative study between perilymph (post mortem), CSF and blood serum



Acta Oto-Laryngologica 106(1-2): 117-123



The large differences in the protein content of perilymph and serum as well as the perilymph volume limit of 10-15 microliter cause serious problems when collecting samples of uncontaminated perilymph in order to analyse its protein composition. In 8 out of 24 extremely carefully taken samples of perilymph removed during autopsy, we were able, by keeping to certain selection criteria, to carry out electrophoretic analyses which were suitable for reproduction and comparison. Comparison of human perilymph, CSF and serum, using SDS-Page and Western blotting, gave the expected agreement which had been suggested by tests on guinea pigs. However, a closer examination of individual proteins pointed to some relatively large differences in quantity and quality.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 041109974

Download citation: RISBibTeXText

PMID: 3421092

DOI: 10.3109/00016488809107378


Related references

Protein profile of human perilymph: in search of markers for the diagnosis of perilymph fistula and other inner ear disease. Otolaryngology--Head and Neck Surgery 111(3 Pt 1): 273-280, 1994

Mala e dehydrogenase in post mortem perilymph. Acta Oto-Laryngologica 70(5): 336-339, 1970

Malate Dehydrogenase in post Mortem Perilymph. Acta Oto-Laryngologica 70(5-6): 336-339, 1970

Pharmacokinetics of amikacin in the perilymph and blood serum an approach to a comparative estimation of potential ototoxicity of aminoglycoside antibiotics. Antibiotiki i Meditsinskaya Biotekhnologiya 31(12): 909-913, 1986

Pharmacokinetics of amikacin in the perilymph and blood serum. An approach to the comparative evaluation of the potential ototoxicity of aminoglycoside antibiotics. Antibiotiki i Meditsinskaia Biotekhnologiia 31(12): 909-913, 1986

Concentrations of inorganic ions in guinea-pig inner ear fluids. II. Post-mortem changes in the ionic composition of utricular endolymph and perilymph. Journal of Laryngology and Otology 80(9): 885-889, 1966

Perilymph fistula: diagnosis by detection of perilymph in the middle ear by beta-2 transferrin immunofixation. Annales d'Oto-Laryngologie et de Chirurgie Cervico Faciale 111(4): 180-184, 1994

Effects of acoustic overstimulation and perilymph perfusion--with Joro spider toxin on the concentrations of amino acids in the perilymph. Nihon Jibiinkoka Gakkai Kaiho 96(2): 231-237, 1993

Perilymph sampling from the cochlear apex: a reliable method to obtain higher purity perilymph samples from scala tympani. Journal of Neuroscience Methods 153(1): 121-129, 2005

Effect of pentobarbital anesthesia on amikacin concentrations in plasma and perilymph and evaluation of multiple sampling in perilymph of guinea pigs. Antimicrobial Agents and ChemoTherapy 27(5): 720-723, 1985

A contribution to the physiology of the perilymph part 1 the origins of perilymph. Annals Of Otology Rhinology & Laryngology: 76-83, 1974

A contribution to the physiology of the perilymph. 1. The origins of perilymph. Annals of Otology, Rhinology, and Laryngology 83(1): 76-83, 1974

Serum and amino acids in the human perilymph. Archiv für Klinische und Experimentelle Ohren- Nasen- und Kehlkopfheilkunde 205(2): 182-186, 1973

Electrophoretic examination of protein distribution in guinea pig perilymph. Acta Oto-Laryngologica 81(1-2): 68-72, 1976

Serum proteins and amino acids in the human perilymph. Archiv fuer Klinische und Experimentelle Ohren- Nasen- und Kehlkopfheilkunde 205(2): 182-186, 1973