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Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells


, : Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells. Proceedings of the National Academy of Sciences of the United States of America 88(23): 10480-4

The particulate enzyme responsible for the synthesis of endothelium-derived relaxing factor has been purified from cultured and native (noncultured) bovine aortic endothelial cells. Purification of the solubilized particulate enzyme preparation by affinity chromatography on adenosine 2',5'-bisphosphate coupled to Sepharose followed by Superose 6 gel filtration chromatography resulted in a single protein band after denaturing polyacrylamide gel electrophoresis that corresponded to approximately 135 kDa. The enzyme activity in the various fractions was assayed by its stimulatory effect on soluble guanylyl cyclase of rat fetal lung fibroblasts (RFL-6 cells), by the formation of L-citrulline from L-arginine, by measuring nitrite/nitrate formation, and by bioassay on endothelium-denuded vascular strips. Endothelium-derived relaxing factor synthase was purified 3419-fold from the crude particulate fraction of cultured bovine aortic endothelial cells with a 12% recovery (RFL-6 assay). Purified endothelium-derived relaxing factor synthase required L-arginine, NADPH, Ca2+, calmodulin, and 5,6,7,8-tetrahydrobiopterin for full activity.

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Accession: 041135330

PMID: 1720542

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Novy, M.J.; Walsh, S.W.; Kittinger, G.W., 1977: Experimental fetal anencephaly in the rhesus monkey: effect on gestational length and fetal and maternal plasma steroids. Journal of Clinical Endocrinology and Metabolism 45(5): 1031-1038

Cleare, Jr., L. D., 1928: A Method for the Bearing of Egg Parasites of the Sugar-cane Moth Borers. A detailed account is given of a method and apparatus employed in British Guiana since 1921 for the rearing of the egg parasites of the small moth borers of sugar-cane (Diatraea spp.). Diagrams are given showing the construction of the cages used,...

Trayhurn P EDITOR-IN-CHIEF, P., 2004: Recent highly cited articles in the British Journal of Nutrition (including Supplements): An update. British Journal of Nutrition 92(1): 1-3

Osborne, M. C.; Dunbar, R. B.; Mucciarone, D. A.; Sanchez-Cabeza, J., 2009: Coral delta18 O stratigraphy from the West Pacific Warm Pool, Palau. Eos, Transactions, American Geophysical Union 90.52, Suppl

Ozdemir, H.; Ciftçi, E.; Durmaz, Rıza.; Güriz, H.; Aysev, A.Derya.; Karbuz, A.; Gökdemir, R.; Acar, Bülent.; Nar Ötgün, S.; Ertek, M.; Köse, S.Kenan.; Ince, E., 2015: Nasopharyngeal carriage of Streptococcus pneumoniae in healthy Turkish children after the addition of PCV7 to the national vaccine schedule. European Journal of Pediatrics 173(3): 313-320

Ramos Lopes, M.M., 1988: Cardiovascular prevention: reality or myth?. Arquivos Brasileiros de Cardiologia 50(4): 219-221

Pennec, Y.; Horn von Hoegen, M.; Zhu, X.; Fortin, D.C.; Freeman, M.R., 2006: Dynamics of an Ising chain under local excitation: a scanning tunneling microscopy study of Si(100) dimer rows at 5 K. An extension of the classical Ising model to a situation including a source of spin-flip excitations localized on the scale of individual spins is considered. The scenario is realized by scanning tunneling microscopy of the Si(100) surface at low...

Milano, G.; Grasso, A.; Salvatore, M.; Saccomanno, M.F.; Deriu, L.; Fabbriciani, C., 2011: Arthroscopic rotator cuff repair with metal and biodegradable suture anchors: a prospective randomized study. Arthroscopy 26(9 Suppl): S112-S119

Cattaneo, A.D.; Launo, C.; Bassi, C.; Molinino, M., 1982: Rapid tracheal intubation with fazadinium. Minerva Anestesiologica 48(3): 145-154

Gallacchi G., 1989: Pain therapy in rheumatology with thermotherapy and cryotherapy. Kaganas, G , W Mueller And F J Wagenhaeuser (Ed ) Fortbildungskurse Fuer Rheumatologie, Band 8 Schmerzbehandlung in Der Rheumatologie; (Continuing Education Courses in Rheumatology, Vol 8 Pain Therapy in Rheumatology); Meeting Xii+241p S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus Paper 94-105