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Purification and properties of two molecular forms of arginine kinase from the adductor muscle of the scallop, Pecten maximus


Purification and properties of two molecular forms of arginine kinase from the adductor muscle of the scallop, Pecten maximus



Comparative Biochemistry and Physiology. B Comparative Biochemistry 99(2): 387-394



ISSN/ISBN: 0305-0491

PMID: 1764917

DOI: 10.1016/0305-0491(91)90059-m

1. Two molecular forms of arginine kinase, AK1 and AK2 have been purified from the adductor muscle of the scallop, Pecten maximus. AK2 was retained on a DEAE-cellulose column at pH 7.5, but AK1 was not. 2. Both forms were monomeric (mol. wt. approximately 42,000) and showed the same pH optimum (7.5-8.0) in the direction of phosphoarginine synthesis. 3. AK1 had slower electrophoretic mobility at pH 8.3 towards the anode, higher lysine content, lower glutamate content, lower Km for L-arginine and higher Km for Mg(2+)-ATP than AK2. Unlike AK1, AK2 was strongly inhibited at high concentrations of Mg(2+)-ATP. 4. Both molecular forms cross-reacted with antisera raised against native as well as performic acid-oxidized lobster muscle arginine kinase. However, AK1 showed a greater affinity than AK2 to anti-lobster arginine kinase antibodies, particularly to those raised against the native enzyme.

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Accession: 041137150

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