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Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye

Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye

Analytical Biochemistry 193(2): 231-235

PCR primers covalently labeled with biotin and a fluorescent dye allow immobilization and separation of the products which can be quantitatively analyzed subsequently. The procedure we have developed circumvents electrophoretic separation and radioactive labeling. Exact quantitative analysis of reaction products is feasible during the logarithmic phase of amplification when Taq polymerase is not limiting, as it is during the plateau phase of the reaction. With appropriate standardization the procedure can be used for routine diagnostic purposes.

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Accession: 041148221

Download citation: RISBibTeXText

PMID: 1908194

DOI: 10.1016/0003-2697(91)90014-k

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