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RNA processing by RNase IIi is involved in the synthesis of Escherichia coli polynucleotide phosphorylase

RNA processing by RNase IIi is involved in the synthesis of Escherichia coli polynucleotide phosphorylase

Molecular and General Genetics: Mgg 209(1): 28-32

ISSN/ISBN: 0026-8925

PMID: 2823071

The synthesis of Escherichia coli polynucleotide phosphorylase (PNPase) was examined in a mutant strain defective in the RNA processing enzyme RNase III (Rnc-). We found that the specific activity and the synthesis rate of PNPase were increased in the Rnc- strain by more than three times that in an Rnc+ strain. Such increased synthesis of PNPase was not observed in a mutant strain transformed with a plasmid carrying the rnc+ gene. Quantitative analysis of RNA showed that the transcripts from the pnp gene, which encodes PNPase, were degraded more slowly in the Rnc- strain than in the Rnc+ strain. These results indicate that processing of the transcripts by RNase III is intimately involved in controlling the expression of pnp by affecting the stability of its messenger RNA.

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Accession: 041155775

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