+ Translate
+ Most Popular
Allopurinol and Loss of Consciousness in a 78-old Year Man Suffering from Gout
The parasites of British birds and mammals. 3XIV-3XVH
Studies on the in vitro synthesis of phi x 174 super helical covalently closed circular duplex dna and circular single stranded dna
The Shahada movement: a peasant movement in Maharashtra (India) - its development and its perspectives
The cranial nerves of Wallago attu
Anatomy of Mystus seenghala IV Nervous system
Haematology of Persian fallow deer (Dama mesopotamica)
Major weeds of rabi and kharif crops and their control
Structure of Morija-Banol iron-ore deposits, Rajasthan, and its control over mineralization
Donkioporia expansa - A lesser known wood destroyer in buildings
The new physiology of vision 3Xin. The testing of color-vision
Economic importance of Pteridophytes
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Ketoconazole shampoo 1% vs. climbazole shampoo in the treatment of seborrhoeic scalp dermatitis
Economic efficiency of biwall drip irrigation in sugarcane production - a case study in Ankalkhop village in Sangli District of Maharashtra
48,4X-49,5X mosaic: asynchronies among the late-replicating X chromosomes
Compatibility of potassium phosphonate (Akomin-40) with different species of Trichoderma and Gliocladium virens
Enterobacter amnigenus. An unusual human pathogen
The 48 4X 49 4Xy 49 4X iso chromosome yq mosaicism in a 3 year old boy from a twin pregnancy
Placentrex treatment in corneal defects in animals
The biology of human starvation: some new insights
Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA
Replenishable but depletable resources: water
New insights into bestiality and zoophilia
Thoracic outlet syndrome in Ehlers-Danlos patients as displayed by magnetic resonance imaging, angiography and venography

Rat liver plasma membrane Ca2+- or Mg2+-activated ATPase. Evidence for proton movement in reconstituted vesicles

Rat liver plasma membrane Ca2+- or Mg2+-activated ATPase. Evidence for proton movement in reconstituted vesicles

Biochimica et Biophysica Acta 904(2): 251-258

ISSN/ISBN: 0006-3002

PMID: 2822118

DOI: 10.1016/0005-2736(87)90374-9

The Ca2+- or Mg2+-activated ATPase from rat liver plasma membrane was partly purified by treatments with sodium cholate and lysophosphatidylcholine, and by isopycnic centrifugation on sucrose gradients. The ATPase activity had high sensitivity to detergents, poor nucleotide specificity and broad tolerance for divalent cations. It was insensitive to mitochondrial ATPase inhibitors such as oligomycin and to transport ATPase inhibitors such as vanadate and ouabain. Using the cholate dialysis procedure, the partly purified enzyme was incorporated into asolectin vesicles. Upon addition of Mg2+-ATP, fluorescence quenching of 9-amino-6-chloro-2-methoxyacridine (ACMA) was observed. The quenching was abolished by a protonophore, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). Asolectin vesicles or purified ATPase alone failed to promote quenching. These data suggest that the Ca2+- or Mg2+-activated ATPase from rat liver plasma membrane is able of H+-translocation coupled to ATP hydrolysis.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 041174984

Download citation: RISBibTeXText

Related references

Proton pumping by vesicles reconstituted from 2 fractions of solubilized rose cell plasma membrane atpase. Biochimica et Biophysica Acta 778(1): 229-232, 1984

ATP-dependent Proton Pumping Catalyzed by the Purified Yeast Plasma Membrane ATPase Reconstituted into Phospholipid Vesicles. Biophysical Journal 37(1): 96-97, 1982

Active proton uptake in lipid vesicles reconstituted with the purified yeast plasma membrane ATPase. Fluorescence quenching of 9-amino-6-chloro-2-methoxyacridine. Journal of Biological Chemistry 257(16): 9365-9371, 1982

Electrogenic H+ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme. Journal of Biological Chemistry 259(12): 7884-7892, 1984

Auxin effects on proton accumulation by reconstituted plasma membrane proton atpase. Phytochemistry (Oxford) 30(5): 1391-1396, 1991

Characterization of native and reconstituted plasma membrane proton atpase from the plasma membrane of beta vulgaris. Journal of Membrane Biology 79(3): 245-256, 1984

Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H(+)-ATPase reconstituted into vesicles. Biochimica et Biophysica Acta 1609(1): 71-79, 2003

Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane proton atpase ii. stimulation of the proton atpase in a plasma membrane fraction purified by phase partitioning. Botanica Acta 104(4): 265-271, 1991

Auxin effects on proton accumulation by reconstituted plasma membrane H+-ATPase. Phytochemistry 1991(5): 1391-1395, 1991

A method for estimating d generated by the H+-ATPase ofSchizosaccharomyces pombein reconstituted plasma membrane vesicles. Folia Microbiologica 39(6): 539-541, 1994

Proton and potassium translocations in reconstituted yeast plasma membrane atpase proteo liposomes. Archives Internationales de Physiologie et de Biochimie 89(5): B211, 1981

Proton translocation catalyzed by the purified yeast plasma membrane atpase reconstituted in liposomes. S Letters: 351-354, 1981

Fusicoccin stimulates the plasma membrane proton atpase in membrane vesicles from various plant materials. Plant Physiology 86(4 Suppl): 140, 1988

Energy dependent hydrogen ion accumulation catalyzed by the yeast plasma membrane atpase reconstituted in phospho lipid vesicles. Archives Internationales de Physiologie et de Biochimie 89(3): B103, 1981

Ion-dependent generation of the electrochemical proton gradient delta muH+ in reconstituted plasma membrane vesicles from the yeast Metschnikowia reukaufii. Biochimica et Biophysica Acta 905(2): 287-294, 1987