Recognition of several idiotopes on a monoclonal anti-hepatitis B virus surface antigen using monoclonal anti-idiotypic antibodies

Thanavala, Y.M.; Moore, M.J.; Tedder, R.; Hay, F.C.; Roitt, I.M.

Immunology 63(4): 575-577


ISSN/ISBN: 0019-2805
PMID: 3259202
Accession: 041188171

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A monoclonal murine antibody H3F5 directed to the a determinant of hepatitis B surface antigen (HBsAg) was used to raise several monoclonal anti-idiotypes. Cross-blocking experiments between the anti-idiotypes and the patterns of inhibition produced by a number of other monoclonal anti-HBsAg, generated in the same fusion as H3F5, identified three idiotopes on H3F5 which were shared to varying degrees with the other anti-HBsAg monoclonals. One behaved as a dominant cross-reacting idiotope (CRI) in that it appeared strongly in 5/7 monoclonal idiotypes. The CRI could represent an important target for regulation by anti-idiotope. Monoclonal antibodies have many advantages over polyclonal sera in the detailed analysis of idiotope structures.