+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reduction of adenylyl cyclase activity by cholera toxin in myeloid cells. Long-term down-regulation of Gs alpha subunits by cholera toxin treatment



Reduction of adenylyl cyclase activity by cholera toxin in myeloid cells. Long-term down-regulation of Gs alpha subunits by cholera toxin treatment



Febs Letters 267(2): 221-225



In IPC-81 cells, the adenylyl-cyclase activation by cholera toxin produces an elevation of cAMP that causes a rapid cytolysis. A resistant clone with deficient cholera toxin-induced cyclase activity (yet sensitive to cAMP) showed a rapid decrease in the amount of membrane-bound Gs alpha (42-47 kDa) detectable soon after ADP-ribosylation of these proteins; pertussis toxin-sensitive G proteins (41 kDa) were not affected. Resistant cells showed a rapid decrease of Gs alpha that is consistent with the finding that cAMP did not accumulate in these cells. Cholera toxin treatment of resistant cells had long-lasting effects (several weeks) on the level of Gs alpha in the cell membrane. The duration of Gs alpha decrease does not correspond to the probable life of catalytically active cholera toxin in the cells, and suggests a regulated process more complex than a proteolytic degradation targeted on ADP-ribosylated molecules.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041200052

Download citation: RISBibTeXText

PMID: 2379582


Related references

Reduction of adenylyl cyclase activity by cholera toxin in myeloid cells down regulation of gsa subunits during ct treatment. Journal of Cellular Biochemistry Suppl. (14 Part C): 151, 1990

Protection against experimental cholera in the rat. A study on the formation of antibodies against cholera toxin and desensitization of adenylate cyclase after immunization with cholera toxin. International Archives of Allergy and Applied Immunology 75(2): 143-148, 1984

Both cholera toxin-induced adenylate cyclase activation and cholera toxin biological activity are inhibited by antibodies against related synthetic peptides. Proceedings of the National Academy of Sciences of the United States of America 81(24): 7893-7896, 1984

Effects of cholera toxin on lymphoid system. I. Reduction of thymus cells after cholera toxin injection. Journal of Clinical and Laboratory Immunology 6(2): 181-183, 1981

The adjuvant effect of cholera toxin cholera toxin b subunit and glutaraldehyde modified cholera toxin on the mucosal immune response of rabbits to 2 acetylaminofluorene thyroglobulin conjugates. FASEB Journal 4(3): A500, 1990

Cholera toxin zonula occludens toxin and accessory cholera enterotoxin gene-negative Vibrio cholerae non-01 strains produce the new cholera toxin. Current Science (Bangalore) 70(12): 1041-1043, 1996

Partial purification of a water-soluble liver protein that regulates adenylate cyclase activity (basal, hormone- and cholera-toxin-activated) and cholera-toxin-catalyzed ADP-ribosylation of the membrane G protein. Biochimica et Biophysica Acta 801(3): 325-333, 1984

Thermotropic behavior and dynamics of cholera toxin and cholera toxin subunits bound to phospho lipid vesicles containing ganglioside gm 1. Biophysical Journalpart 2: 359a, 1984

Properties of the cholera toxin stimulated adenylate cyclase of toad and rat erythrocyte plasma membranes and the mechanism of action of cholera toxin. Federation Proceedings 33(5 Part 2): 1357, 1974

Interaction of cholera toxin and toxin derivatives with lymphocytes. III. Modulating effects in vivo by cholera toxin on the graft-versus-host reactivity of lymphoid cells: suggested inhibition of suppressor cells. International Archives of Allergy and Applied Immunology 57(4): 364-374, 1978

Cholera toxin-mediated ADP-ribosylation of Gsalpha and activation of adenylyl cyclase occurs at the plasma membrane of CaCo-2 cells. Molecular Biology of the Cell 9(Suppl. ): 407A, 1998

Unique properties of the follicle-stimulating hormone- and cholera toxin-sensitive adenylyl cyclase of immature granulosa cells. Endocrinology 118(1): 302-311, 1986

Influence of the A and B subunits of cholera toxin (CT) and Escherichia coli toxin (LT) on TNF-alpha release from macrophages. Toxicon 53(5): 570-577, 2009

Parathyroid hormone stimulates adenylyl cyclase via a cholera toxin sensitive mechanism in cultured rat vascular smooth muscle cells. Journal of the American Society of Nephrology 2(3): 454, 1991

Evaluation of purified cholera toxins and toxoids as immunizing agents against cholera and development of a new technique for measuring cholera toxin toxoid and anti toxin. U S Government Research & Development Reports 71(5): 43, 1971