+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Reevaluation of the 9 compounds reported conclusive positive in yeast Saccharomyces cerevisiae aneuploidy test systems by the Gene-Tox Program using strain D61.M of Saccharomyces cerevisiae

Reevaluation of the 9 compounds reported conclusive positive in yeast Saccharomyces cerevisiae aneuploidy test systems by the Gene-Tox Program using strain D61.M of Saccharomyces cerevisiae

Mutation Research 260(2): 165-180

ISSN/ISBN: 0027-5107

PMID: 2046697

DOI: 10.1016/0165-1218(91)90005-7

The state of aneuploidy test methodology was appraised by the U.S. Environmental Protection Agency in 1986 in analyzing published data. In Saccharomyces cerevisiae 9 chemicals were reported to be conclusive positive for aneuploidy induction in either mitotic or meiotic cells. We reevaluated these 9 chemicals using Saccharomyces cerevisiae D61.M, a strain that detects mitotic chromosome malsegregation. Acetone (lowest effective dose (LED): 40 microliters/ml), bavistan (LED: 5 micrograms/ml), benomyl (LED: 30 micrograms/ml) and oncodazole (LED: 4 micrograms/ml) induced a dose-dependent increase in the frequencies of chromosomal malsegregation. Ethyl methanesulfonate (EMS; highest tested dose (HTD): 1000 micrograms/ml) and methyl methanesulfonate (MMS; HTD: 100 micrograms/ml) did not induce malsegregation but were both potent inducers of other genetic events, detected by an increase in the frequencies of cyhR cells. No increases in both endpoints (malsegregation and other genetic events) were observed after treatment of S. cerevisiae D61.M with cyclophosphamide (CP; HTD: 16 mg/ml) in the absence of S9, p-D,L-fluorophenylalanine (p-FPA; HTD: 250 micrograms/ml) and phorbol-12-myristate-13-acetate (TPA; HTD: 50 micrograms/ml). A marginal increase in the frequency of mitotic chromosome malsegregation was obtained with cyclophosphamide in the presence of S9. Thus our test results largely disagree with those previously published by various authors and taken as conclusive by EPA. We interpret the discrepancies to be due to lack of properly controlled testing (e.g., no check for multiple mutational events). Only with a careful test design it is possible to discriminate between chemicals inducing only chromosome loss and no other genetic effects (e.g., acetone, oncodazole), chemicals inducing a variety of genetic damage but no chromosome loss (e.g., EMS, MMS) and chemicals inducing neither chromosome loss nor other genetic events in yeast (e.g., TPA, p-FPA).

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 041201565

Download citation: RISBibTeXText

Related references

Tests for the induction of mitotic aneuploidy in the yeast saccharomyces cerevisiae strain d 61.m. Ashby, J Et Al (Ed ) Progress in Mutation Research, Vol 5 Evaluation Of Short-Term Tests For Carcinogens: Report Of The International Programme on Chemical Safety's Collaborative Study on in Vitro Assays X+752p Elsevier Science Publishers B V : Amsterdam, Netherlands (Dist in U S And Canada By Elsevier Science Publishing Company, Inc : New York, N Y , Usa) Illus 235-242, 1985

Factors influencing the formations of a yeast head by brewing yeasts saccharomyces cerevisiae saccharomyces cerevisiae saccharomyces carlsbergensis. Journal of the Institute of Brewing 75(2): 200-204, 1969

Genetic effects of procarbazine in the yeast Saccharomyces cerevisiae, strain D4 - Effets génétiques de la procarbazine chez la levure Saccharomyces cerevisiae, souche D4. Teratogenesis, Carcinogenesis, and Mutagenesis 4(2): 201-210, 1984

A study on expression of mammalian g protein gene in saccharomyces cerevisiae construction of chimeric genes of rat gs alpha and yeast scg1 for expression in saccharomyces cerevisiae. Korean Journal of Biochemistry 20(1): 9-18, 1988

Expression of a cloned saccharomyces cerevisiae gene ura 1 is controlled by a bacterial promoter in escherichia coli and a yeast promoter in saccharomyces cerevisiae. Gene 15(2-3): 127-138, 1981

Messung der elektrophoretischen Beweglichkeit von Hefezellen (Saccharomyces cerevisiae) unter kontinuierlicher Chemostat-Fermentation - Mesure de la mobilité électrophorétique de levure (Saccharomyces cerevisiae) lors de fermentation continue en chémostat - Measurement of electrophoretic mobility of yeast cells (Saccharomyces cerevisiae) under continous chemostat fermentation. Chemieingenieurtechnik 60(10): 794-795, 1988

Obtention par recombinaison intra-génomique de clones non-moussants à partir d'une souche de levure d'intérêt oenologique Saccharomyces cerevisiae - Obtention by intragenomic recombination of non-foaming clones from an enological strain of yeast Saccharomyces cerevisiae. Sciences des Aliments 9(2): 253-265, 1989

Evaluation of methods of extraction and partial characterization of cell wall carbohydrates of brewer's yeast strain, Saccharomyces cerevisiae - Evaluation des méthodes d'extraction et caractérisation partielle des glucides de la paroi cellulaire de la levure de bière Saccharomyces cerevisiae. Mircen Journal of Applied Microbiology and Biotechnology 2(3): 383-388, 1986

Zur kosmetischen Wirkung eines Extraktes aus der Hefe Saccharomyces cerevisiae - Cosmetic effect of an extract of the yeast Saccharomyces cerevisiae. Sofw. Seifen, Ole, Fette, Wachse 123(5): 318-325, 1997

Biosynthèse et transport des stérols chez la levure Saccharomyces cerevisiae - Sterol biosynthesis and trafficking in the yeast Saccharomyces cerevisiae. Journee Claude Bernard 192(5): 977-990, 1998

A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast i. isolation and characterization of fusant between saccharomyces cerevisiae and saccharomyces diastaticus. Korean Journal of Applied Microbiology and Bioengineering 14(4): 305-310, 1986

Gewinnung synchron wachsender und sich vermehrender Hefezellen ( Saccharomyces cerevisiae) / Preparation of Synchronously Growing and Multiplying Yeast Cells (Saccharomyces cerevisiae). Zeitschrift für Naturforschung C 30(3-4): 245-247, 1975

The Interaction between Saccharomyces cerevisiae and Non-Saccharomyces Yeast during Alcoholic Fermentation Is Species and Strain Specific. Frontiers in Microbiology 7: 502, 2016

A strain of Saccharomyces cerevisiae which grows efficiently on starch - Une souche de Saccharomyces cerevisiae croissant sur de l'amidon. British Yeast Group Meeting (1983) 7(5): 220-224, 1985

Prüfung von Abwasser auf aquatische Toxizität mit einem Gärungstest der Hefe Saccharomyces cerevisiae - Testing the aquatic toxicity of sewage by a fermentation assay with the yeast Saccharomyces cerevisiae. Vom Wasser 95: 97-106, 2000