+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Regional localization of virus in the central nervous system of mice persistently infected with murine coronavirus JHM



Regional localization of virus in the central nervous system of mice persistently infected with murine coronavirus JHM



Virology 166(2): 328-338



Suckling C57BL/6 mice infected with mouse hepatitis virus strain JHM (MHV-JHM) develop either a fatal acute encephalomyelitis or a late onset demyelinating disease, depending on whether they are nursed by unimmunized or immunized dams. To determine the localization of virus-specific RNA, serial sections of brains from infected and uninfected mice were annealed with a 35S-labeled antisense RNA probe and analyzed by film autoradiography. In the mice with acute encephalomyelitis, viral RNA was present in the mesencephalon, hypothalamus, hippocampus, basal ganglia, subcortical white matter, and thalamus. Viral RNA was detected in the spinal cords of all mice with the late onset, demyelinating encephalomyelitis, but was distributed into three different patterns in the brains of these mice, even though all had the same clinical disease. In the first group, viral RNA was detected only in the brainstem. In the second group, viral RNA was detected in the brainstem, thalamus, and cerebral grey matter. This distribution was consistent with viral spread along well-defined tracts connecting these parts of the brain. In the third group, viral RNA could be detected both in the brainstem and in several white matter tracts within close physical proximity to the optic chiasm. This distribution was consistent with viral spread by an extracellular route from one white matter tract to other tracts which were physically close, but which were not part of the same pathways. These results suggest that MHV-JHM spreads through the central nervous system both along well-defined neuronal pathways and by spread from contiguous structures, but also suggest that viral replicates preferentially in a limited number of areas of the brain. The technique of in situ hybridization with film autoradiography should be generally useful for analyzing macroscopic movements of virus within infected organs.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 041206546

Download citation: RISBibTeXText

PMID: 2845647

DOI: 10.1016/0042-6822(88)90503-x


Related references

Location and distribution of virus antigen in the central nervous system of mice persistently infected with Theiler's virus. British Journal of Experimental Pathology 64(1): 57-65, 1983

High-magnitude, virus-specific CD4 T-cell response in the central nervous system of coronavirus-infected mice. Journal of Virology 75(6): 3043-3047, 2001

Theilers virus isolated from a persistently infected oligodendroglioma cell line fails to induce demyelinating disease in central nervous system of sjl j mice. Society for Neuroscience Abstracts 15(2): 1385, 1989

Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM. Viral Immunology 23(4): 437-442, 2010

Infection of central nervous system cells by ecotropic murine leukemia virus in C58 and AKR mice and in in utero-infected CE/J mice predisposes mice to paralytic infection by lactate dehydrogenase-elevating virus. Journal of Virology 69(1): 308-319, 1995

The murine coronavirus mouse hepatitis virus strain A59 from persistently infected murine cells exhibits an extended host range. Journal of Virology 71(12): 9499-9507, 1997

The course of disease and persistence of virus in the central nervous system varies between individual CBA mice infected with the BeAn strain of Theiler's murine encephalomyelitis virus. Journal of General Virology 77: 2701-2711, 1996

Apoptosis of infiltrating T cells in the central nervous system of mice infected with Theiler's murine encephalomyelitis virus. Virology 315(1): 0-23, 2003

Selection of CTL escape mutants in mice infected with a neurotropic coronavirus: quantitative estimate of TCR diversity in the infected central nervous system. Journal of Immunology 163(11): 6106-6113, 1999

Characterization of and functional antigen presentation by central nervous system mononuclear cells from mice infected with Theiler's murine encephalomyelitis virus. Journal of Virology 72(10): 7762-7771, 1998

Differential antigen recognition by T cells from the spleen and central nervous system of coronavirus-infected mice. Virology 222(1): 247-251, 1996

Immunohistological detection of bovine viral diarrhoea virus antigen in the central nervous system of persistently infected cattle using monoclonal antibodies. Veterinary Microbiology 23(1-4): 203-210, 1990

Murine coronavirus spike glycoprotein mediates degree of viral spread, inflammation, and virus-induced immunopathology in the central nervous system. Virology 301(1): 9-20, 2002

Murine coronavirus receptors are differentially expressed in the central nervous system and play virus strain-dependent roles in neuronal spread. Journal of Virology 84(21): 11030-11044, 2010

Viral antigen distribution in the central nervous system of cattle persistently infected with bovine viral diarrhea virus. Veterinary Pathology 26(1): 26-32, 1989