+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of genes encoding the large subunit of ribulose-1,5-bisphosphate carboxylase and the photosystem II polypeptides D-1 and D-2 during the cell cycle of Chlamydomonas reinhardtii

, : Regulation of genes encoding the large subunit of ribulose-1,5-bisphosphate carboxylase and the photosystem II polypeptides D-1 and D-2 during the cell cycle of Chlamydomonas reinhardtii. Journal of Cell Biology 103(5): 1837-1845

Synthesis of the major chloroplast proteins is temporally regulated in light-dark-synchronized Chlamydomonas cells. We have used cloned chloroplast DNA probes, and in vitro and in vivo protein synthesis to examine the cell cycle regulation of photosystem II polypeptides D-1 and D-2, and the large subunit of ribulose-1,5-bisphosphate carboxylase (RuBPCase LS). Synthesis and accumulation of D-1 and D-2 mRNAs occurs during the first half of the light period (G1), correlating with increasing synthesis of the polypeptides. Rifampicin, added immediately before the light period, inhibited the normal increase in D-1, D-2 polypeptide synthesis. During the dark period D-1, D-2 mRNAs persist at high levels despite reduced rates of mRNA synthesis and translation during this period. Cell-free translation analyses indicate that the D-1 mRNA present during the dark period is efficient at directing synthesis of the D-1 precursor in vitro. We conclude that expression of the psbA (D-1) and psbD (D-2) genes are regulated primarily at the transcriptional level during the light-induction period but at the translational level for the remainder of the cell cycle. Transcripts of the RuBPCase LS gene (rbcL) are also found at high levels during the light and dark periods but, unlike D-1 and D-2, LS mRNA levels do not increase until the last half of the light period and measurable synthesis and accumulation of this mRNA occurs during the dark. Furthermore, induction of LS polypeptide synthesis during the light period is insensitive to rifampicin. We conclude that LS production is regulated primarily at the translational level during the cell cycle.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 041210674

PMID: 3536953

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Frucht-Pery, J.; Feldman, S.T., 1993: Cataract surgery in a leprosy population in Liberia. In Liberia, 43 eyes of 30 patients with ocular leprosy underwent cataract extraction; 33 eyes had extracapsular cataract extraction (ECCE) and 10 eyes ahd intracapsular cataract extraction (ICCE). ICCE was performed in eyes with poor visualization...

Neumayer, L., 1895: Die Grosshirnrinde der niederen Vertebraten. Sitzungsberichte Ges Munchen, XI 60-70

Nedin, Christopher.; Jenkins, Richard JF., 1998: First occurrence of the Ediacaran fossil Charnia from the southern hemisphere. Alcheringa, 223-4: 315-316

Lemm, Ca, 1983: Growth and survival of Atlantic salmon fed semimoist or dry starter diets Salmo salar. Progressive fish culturist United States Fish and Wildlife Service 45(2): 72-75

Hershey H.P.; Colbert J.T.; Lissemore J.L.; Barker R.F.; Quail P.H., 1984: Molecular cloning of complementary dna for avena sativa phytochrome. Several c[complementary]DNA clones for phytochrome, a plant regulatory photoreceptor, were isolated. A cDNA library was constructed by using etiolated Avena poly(A)+ RNA enriched for phytochrome mRNA by size fractionation. Replicate arrays of colo...

Huri, E.; Ayyildiz, A.; Nuhoğlu, B.; Germiyanoğlu, C., 2007: Spontaneous rupture and emergency repairment of the renal pelvis. A case of spontaneous rupture of the renal pelvis is very rare and unusual occurrence. Our case was a young woman who did not present with any disease that was a factor for pelvic rupture. The diagnosis was confirmed by abdomino-pelvic CT, and em...

Rischkow, V., 1927: New data on veined mottling in Euonymus japonicus and Euon. radicans. The mottling of the veins in the foliage of Euonymusjaponica fol. marmor, investigated by the author at Kharkov, is stated to be due to an infectious chlorosis identical with that described by Baur (Ber. Deutsch. Bot. Gesellsch., xxvi, 1908) as oc...

Patch, E. M., 1917: Eastern Aphlds, new or little known. Part I. The species described in this paper from the Eastern United States include: Aphis viburniphila, sp. n., found on many species of Viburnum; this species is largely parasitized by Hymenoptera; A. rumexi-colens, sp. n., found on Rumex acetosella; A....

Kashiwagi, K.; Shinkai, T.; Kajii, E.; Kashiwagi, A., 2005: The effects of reactive oxygen species on amphibian aging. To clarify the role of reactive oxygen species (ROS) in the aging process of amphibians, antioxidant enzyme activity and indexes of ROS damage were investigated biochemically using the livers of 3- and 10-year-old Rana nigromaculata frog males and...

Yamazaki, Y.; Wakayama, T.; Yanagimachi, R., 2001: Contribution of cumulus cells and serum to the maturation of oocyte cytoplasm as revealed by intracytoplasmic sperm injection (ICSI). The fertilizability and developmental capacity of mouse oocytes matured in vitro were examined by in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). While more than 50% of cumulus-enclosed oocytes were fertilized by IVF afte...