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Regulation of growth and DNA synthesis in mouse lymphoma L5178Y cells under conditions of nutrient depletion


, : Regulation of growth and DNA synthesis in mouse lymphoma L5178Y cells under conditions of nutrient depletion. Archivum Immunologiae et Therapiae Experimentalis 25(1): 149-155

The effect of cultivation of L5178Y lymphoblasts to high density and in two kinds of nutrient-depleted medium on their growth was observed. It was found that in cell population grown to stationary phase the mitotic index and labeled thymidine incorporation dropped about 3 times in comparison with exponential control. Upon dilution with fresh medium the cells resume traverse the life cycle in partially synchronized manner. Cultivation of the cells in medium with 0-5% serum induced very rapid loss of their viability. Cultivation of the cells in medium without asparagine and with dialyzed serum caused significant slowing of their growth-rate and upon dilution with complete medium the cells entered the exponential rate of division in an asynchronous manner.

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Accession: 041210836

PMID: 879995

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