+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of growth and gene activity in euploid hybrids between human neonatal fibroblasts and epithelioid amniotic fluid cells

, : Regulation of growth and gene activity in euploid hybrids between human neonatal fibroblasts and epithelioid amniotic fluid cells. American Journal of Human Genetics 30(4): 392-405

Pure populations of proliferating synkaryons were obtained from polyethylene glycol-mediated crosses between diploid human foreskin fibroblasts and epithelioid amniotic fluid cells. These hybrids proved to be chromosomally stable tetraploids. They continuously produced heteropolymeric G6PD and showed strictly additive patterns of silver staining of both parental sets of nucleolar organizing chromosomes. Collagenous proteins characteristic of the fibroblast parent were synthesized, while fibronectin production appeared to be directed by the epithelioid portion of the genome. Even though these heterotypic hybrids proliferated at a reduced rate and achieved fewer population doublings relative to homotypic (fibroblast X fibroblast) crosses, they survived passage by trypsinization better than pure populations of epithelioid cells. These observations suggest a concerted action of both parental genomes with respect to proteins responsible for "household" functions, but complementation and possibly modulation of gene action with respect to "luxury" protein synthesis and cell growth.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 041210842

PMID: 717401

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Schulze-Osthoff, K.; Los, M.; Baeuerle, P.A., 1995: Redox signalling by transcription factors NF-kappa B and AP-1 in lymphocytes. Biochemical Pharmacology 50(6): 735-741

Jones, H.E.; van de Wijgert, J.H.H.M., 2016: DMPA use and HIV acquisition: time to switch to NET-EN?. Lancet. HIV 2(7): E263-E264

Atkinson J. H., 1993: Engineering geologists in the construction industry. Geoscientist: Pages 9-11. 1993.

Sidorov, F. F.; Cekalin, N. M., 1964: Smut resistance of inbred maize lines. The results of artificial infection of 333 lines showed the popcorns to be more resistant than other types. A list is given of 83 lines found to be more resistant than the rest.

Davidson, L.; Vandongen, R.; Beilin, L.J., 1981: Effects of eating bananas on plasma free and sulfate-conjugated catecholamines. Life Sciences 29(17): 1773-1778

Schraader, E.B.; Hesseling, P.B., 1987: Pleuropericardial effusions in children with non-Hodgkin's lymphoma. A report of 2 cases. Two children with non-Hodgkin's lymphoma (NHL) who presented with pleuropericardial effusions are reported on. Pericardial effusions are very unusual in children with mediastinal nodal NHL. In the first patient, who presented with a pleural e...

Taylor, G.W., 1967: Chronic lymphoedema. British Journal of Surgery 54(10): 898-900

Bowers, MA., 1985: Experimental analyses of competition between two species of bumble bees (Hymenoptera: Apidae). Aspects of flower usage and forager body size in sympatric and experimentally-induced allopatric populations of Bombus flavifrons and B. rufocinctus were studied in 10 discrete subalpine meadows over the last half of one summer in Utah, USA. Resul...

Hopper, W.C., 1979: Genetics in orthopedics. Ona Journal 6(5): 183-185

Anonymous, 1995: The Askov long-term experiments on animal manure and mineral fertilizers. The Askov long term experiments were initiated in 1894 to establish the nutritive value to crops of farmyard manure and to compare the effects of N, P and K fertilizers applied individually, in combinations or as supplement to manure dressings. Va...