EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of human cytotoxic responses by complement: C3, C3b and C3d preparations enhance human allogeneic cytotoxic responses


Journal of Immunopharmacology 8(4): 529-541
Regulation of human cytotoxic responses by complement: C3, C3b and C3d preparations enhance human allogeneic cytotoxic responses
Complement components and complement breakdown products have been found to participate in the regulation of the immune response. In the present study we investigated the effect of C3 and its fragments, C3b, C3c and C3d on human allogeneic cell mediated lympholysis (CML). C3 and C3b at a concentration of 275 M X 10(-9) and C3d at a concentration of 330 M X 10(-9) enhanced human allogeneic CML by at least two fold. In contrast C3c did not affect CML responses. Both C3b and C3d had to be present at the initiation of the cultures in order to exert their effect. Similar doses of C3b and C3d did not affect the mixed lymphocyte responses (3H-thymidine uptake) while higher doses were clearly inhibitory. None of the preparations induced proliferative or cytotoxic responses in the absence of allogeneic stimulating cells. C3b and C3d added to the mixed lymphocyte cultures caused increased production of interleukin 2. We conclude that C3b and C3d facilitate allogeneic cytotoxic responses through increased production of interleukin 2.


Accession: 041211084

PMID: 2949021

DOI: 10.3109/08923978609026504



Related references

Modulation of cytotoxic responses by complement c 3c 3b and c 3d fragments enhance allogeneic cell mediated lympholysis. Federation Proceedings 44(6): 1877, 1985

Complement receptors and cytotoxic responses monoclonal antibodies directed against complement receptor 1 and complement receptor 3 inhibit the generation of human allospecific and virus specific cytotoxic cells in vitro. Journal of Immunopharmacology 8(1): 75-88, 1986

Complement inhibits immune responses: C3 preparations inhibit the generation of human cytotoxic T lymphocytes. European Journal of Immunology 13(4): 279-284, 1983

Complement receptors (CR) and cytotoxic responses: monoclonal antibodies directed against CR1 and CR3 inhibit the generation of human allospecific and virus specific cytotoxic cells in vitro. Journal of Immunopharmacology 8(1): 75-88, 1986

Allogeneic responses of human fetal 22 to 25 week peripheral blood lymphocytes preferential recruitment of cytotoxic effector cells with both cytotoxic t lymphocyte activity and natural killer like function. Cellular Immunology 102(2): 355-363, 1986

Human allogeneic responses: lymphokine requirement for the in vitro generation of specific cytotoxic responses to a malignant melanoma cell line. Australian Journal of Experimental Biology and Medical Science 60(Pt 2): 215-217, 1982

Enhancement of human allogeneic cytotoxic responses by interferons. Journal of Immunopharmacology 7(4): 403-415, 1985

In vitro induction of cytotoxic effector cells against human neoplasms. I. Sensitization conditions and effect of cryopreservation on the induction and expression of cytotoxic responses to allogeneic leukemia cells. Journal of Immunological Methods 28(3-4): 303-319, 1979

Proliferative and cytotoxic responses of human cord blood T lymphocytes following allogeneic stimulation. Cellular Immunology 154(1): 14-24, 1994

Identification of Akt-selective cytotoxic compounds that enhance cytotoxic responses to rapamycin. Cancer Biology & Therapy 10(12): 1256-1261, 2011