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Regulation of human platelet adenylate cyclase by epinephrine, prostaglandin E1, and guanine nucleotides. Evidence for separate guanine nucleotide sites mediating stimulation and inhibition

, : Regulation of human platelet adenylate cyclase by epinephrine, prostaglandin E1, and guanine nucleotides. Evidence for separate guanine nucleotide sites mediating stimulation and inhibition. Journal of Biological Chemistry 254(21): 10791-7

A method for preparing human platelet membranes with high adenylate cyclase activity is described. Using these membranes, epinephrine and GTP individually are noted to inhibit adenylate cyclase slightly. When present together, epinephrine and GTP act synergistically to cause a 50% inhibition of basal activity. The epinephrine effect is an alpha-adrenergic process as it is reversed by phentolamine but not propranolol. The quasi-irreversible activation of adenylate cyclase by Gpp(NH)p is time, concentration, and Mg2+-dependent but is not altered by the presence of epinephrine. Adenylate cyclase activated by Gpp(NH)p, and extensively washed to remove unbound Gpp(NH)p, is inhibited by the subsequent addition of Gpp(NH)p, GTP, and epinephrine. This effect of epinephrine is also an alpha-adrenergic phenomenon. In contrast to epinephrine which inhibits the cyclase, PGE1 addition results in enzyme stimulation. PGE1 stimulation does not require GTP addition. PGE1 accelerates the rate of Gpp(NH)p-induced activation. Low GTP concentrations (less than 1 x 10(-6) M) enhance PGE1 stimulation while higher GTP concentrations cause inhibition. These observations suggest that human platelet adenylate cyclase possesses at least two guanine nucleotide sites, one which interacts with the alpha-receptor to result in enzyme inhibition and a second guanine nucleotide site which interacts with the PGE1 receptor and causes enzyme stimulation.

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Accession: 041211137

PMID: 500609

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Akhmet yev M. A.; Golovneva L. B., 1998: New data on composition and age of Malomikhaylovskay flora, Upper Cretaceous, lower Amur region. Stratigrafiya, Geologicheskaya Korrelyatsiya 6(3): 43-55

Audemard Franck A., 2006: Surface rupture of the Cariaco July 09, 1997 earthquake on the El Pilar Fault, northeastern Venezuela. This paper discusses the surface rupture of the Cariaco July 09, 1997 Ms 6.8 earthquake in northeastern Venezuela--located at 10.545 degrees N and 63.515 degrees W and about 10 km deep. The field reconnaissance of the ground breaks confirms that t...

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Hauser M.S.; Freije S.; Payne R.W.; Timen S., 1989: Bilateral ossifying fibroma of the maxillary sinus. A case involving a 35-year-old man was massive, bilateral, slow-growing ossifying fibromas in the maxillary sinuses resulting in facial deformity and orbital compression is discussed. The historical difficulty in categorizing fibro-osseous lesions...

Crevits, B.; Straeten, E. van der; Naeyaert, J. M.; Hecke, E. van; Kint, A., 1992: Chronic aspergillosis in chronic granulomatous disease. A case of chronic aspergillosis is reported in a 21-yr-old man with chronic granulomatous disease, who presented with a painful swelling of the right big toe. Radiography demonstrated osteolytic lesions in the toes, and the prostate was firm and p...

Fritig, B; Hirth, L; Ourisson, G., 1970: Biosynthesis of the coumarins: scopoletin formation in tobacco tissue cultures. Feeding experiments of tobacco tissue cultures with (U)-14C-phenylalanine, 2-14C-cinnamic acid, 2-14C-glucosidoferulic acid and methyl-14C-methionine were carried out over periods from 3 min to 10 hr. The use of short feedings enabled us to study...

Nathanson, M.H.; Muir, A.; McLaren, G.D., 1985: Iron absorption in normal and iron-deficient beagle dogs: mucosal iron kinetics. Absorption of dietary iron requires uptake of iron by the brush border of the intestinal epithelial cells, intracellular transport, and transfer to the systemic circulation. In iron-deficiency anemia, iron absorption is greatly increased, but the...

Nain J.N., 1975: Symposium on cyclic nucleotides and mammalian cells. Metabolism Clinical & Experimental 24(3): 235-456