EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of intracellular iron distribution in K562 human erythroleukemia cells


, : Regulation of intracellular iron distribution in K562 human erythroleukemia cells. Journal of Biological Chemistry 261(10): 4587-4593

Following a pulse with 59Fe-transferrin, K562 erythroleukemia cells incorporate a significant amount of 59Fe into ferritin. Conditions or manipulations which alter the supply of iron to cells result in changes in the rate of ferritin biosynthesis with consequent variations in the size of the ferritin pool. Overnight exposure to iron donors such as diferric transferrin or hemin increases the ferritin level 2-4- or 6-8-fold above that of the control, respectively. Treatment with the anti-human transferrin receptor antibody, OKT9 (which reduces the iron uptake by decreasing the number of transferrin receptors) lowers the ferritin level by approximately 70-80% with respect to the control. The fraction of total cell-associated 59Fe (given as a pulse via transferrin) that becomes ferritin bound is proportional to the actual ferritin level and is independent of the instantaneous amount of iron taken up. This has allowed us to establish a curve that correlates different levels of intracellular ferritin with corresponding percentages of incoming iron delivered to ferritin. Iron released from transferrin appears to distribute to ferritin according to a partition function; the entering load going into ferritin is set for a given ferritin level over a wide range of actual amounts of iron delivered.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 041211388

PMID: 3457008

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Fineman, R. M.; White, N. J.; Hudspeth, K. L.; Doyle, D. L.; Spice, C.; Bentyelzen Smith, J., 1996: The Washington State genetic education project for primary care provider. American Journal of Human Genetics 59(4 SUPPL ): A6

Kennedy, B. J., 1964: Estrogenic hormone therapy in advanced breast cancer. Ann Internal Med 60(4): 718

Coulter, A. W.; Bueding, E.; Batzinger, R. P., 1977: Differences in anti schistosomal and mutagenic properties between an iso thio cyano di phenylamine and an iso cyano nitro di phenylamine. While 4-isothiocyano-4'-nitrodiphenylamine has high schistosomicidal activity in vivo and is devoid of mutagenic properties in vitro, the reverse is true for the isocyano analogue of this compound; i.e., replacement of the S by O results in a...

Sullivan, R.; Duchesne, C.; Fahmy, N.; Morin, N.; Dionne, P., 1990: Protein synthesis and acrosome reaction-inducing activity of human cumulus cells. The acrosome reaction of human spermatozoa is a prerequisite to fertilization. It has been hypothesized that secretions of cumulus cells could be involved in the induction of this sperm reaction. A source of variation in the in-vitro fertilization...

Richard D. Hey, 1979: Causal and functional relations in fluvial geomorphology. Earth Surface Processes and Landforms 4(2): 179-182

Sugarbaker, Paul H., 1997: Monitoring after resection of upper and lower gastrointestinal cancer. Wanebo, H J [Editor] Surgery for gastrointestinal cancer : 87-94

BELLANTI, J.A.; EITZMAN, D.V.; ROBBINS, J.B.; SMITH, R.T., 1963: The development of the immune response. Studies on the agglutinin response to Salmonella flagellar antigens in the newborn rabbit. By intensive stimulation with large amounts of Salmonella flagellar antigen, newborn rabbits were induced to form high titer flagellar agglutinins usually by the 7th to 10th day of life. Characterization of the agglutinins at various times during...

Privalle, Laura S.; Meghji, Moez; Powell, Luann, 1999: Safety asessment of a novel plant selectable marker Phosphomannose isomerase. Plant Biology (Rockville) : 97

Udias A.; Buforn E., 1988: Single and joint fault-plane solutions from first-motion data. Pages 443-453 1988

Baroffio A.; Raddatz E.; Kucera P., 1985: Insulin increases glucose utilization in the early chick embryo. Experientia (Basel) 41(6): 774