EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of kappa/lambda immunoglobulin light chain expression in normal murine lymphocytes


, : Regulation of kappa/lambda immunoglobulin light chain expression in normal murine lymphocytes. Molecular Immunology 24(7): 751-757

Past work has demonstrated that the kappa:lambda ratio in serum immunoglobulins varies significantly among species. In murine systems it has been established that serum levels of immunoglobulins containing kappa and lambda light chains are present in concentrations of 95 and 5%, respectively. The experiments reported here were designed to determine the molecular basis for this unbalanced ratio. We examined spleens and Peyer's patches (PP) of CBA/J mice for the ratio of kappa to lambda for surface Ig expression, mRNA accumulation, transcription and in vitro translation. Studies of the levels of B-cells expressing kappa and lambda revealed the percentages to be similar to the protein concns in serum (90% kappa +, 10% lambda +). However, further analysis of light chain mRNA accumulation and nuclear transcription revealed ratios of kappa:lambda only slightly greater than 1.0 in most cases. In addition, in vitro translation of B-cell-derived mRNA demonstrated greater translation of kappa chains relative to lambda, though clearly not totally sufficient to provide a serum ratio of 95:5. The results suggest that the low concn of serum lambda relative to kappa in the mouse is most likely due to post-translational events such as inefficient protein processing, poor association with heavy chains, etc. In addition, these data support the hypothesis that lambda-mRNA is present in some kappa-bearing B-cells since lambda-mRNA levels are higher than one would predict from a population of cells in which only 5% express surface lambda chain protein.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 041211461

PMID: 3116408

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Majeroni, B.A.; Ukkadam, S., 2007: Screening and treatment for sexually transmitted infections in pregnancy. Many sexually transmitted infections are associated with adverse pregnancy outcomes. The Centers for Disease Control and Prevention recommends screening all pregnant women for human immunodeficiency virus infection as early as possible. Treatment...

Vallet, A.; Tinet, C., 2013: Characteristics of droplets from single and twin jet air induction nozzles A preliminary investigation. Spray drift from pesticide application is of increasing social concern. Air induction nozzles produce large droplets some of which contain air bubbles, leading to spray drift mitigation and possibly to droplet deposit increase. The purpose of this...

Terayama, N.; Matsui, O.; Gabata, T.; Kobayashi, S.; Sanada, J.; Ueda, K.; Kadoya, M.; Kawamori, Y., 2002: Accumulation of iodized oil within the nonneoplastic liver adjacent to hepatocellular carcinoma via the drainage routes of the tumor after transcatheter arterial embolization. Cardiovascular and Interventional Radiology 24(6): 383-387

Miller, A.B., 1980: Methodologic considerations for trials of lung cancer prophylaxis. Prophylaxis is likely to be of increasing importance in the control of [human] lung cancer. Areas already suggested as potential fields of investigation include the use of specific immunoprophylaxis or synthetic retinoids. Thorough evaluation of t...

Davies, Glenn M.; Dasbach, Erik J.; Shahinfar, Shahnaz; Simpson, Roger; Haffner, Steven M., 2001: Quality of life in patients with NIDDM and renal disease at baseline The losartan renal protection study. Diabetes 50(Supplement 2): A217, June

Ben Haj Khalifa, A.; Khedher, M., 2010: Susceptibility patterns of Acinetobacter baumannii strains isolated in the Mahdia region. Medecine et Maladies Infectieuses 40(2): 126-128

Cermák, J.; Bosák, V., 1970: Heart volume in obese persons. 3. Effect of a marked obesity on the heart volume in men performing physical labor. Archiv für Kreislaufforschung 62(1): 12-24

Thongpriwan, V.; Buseh, A.; Arunothong, W., 2016: Reflections on attitudes, experiences, and vulnerability of intimate partner violence among Southeast Asian college women living in United States. To provide culturally sensitive intimate partner violence (IPV) prevention programs for ethnic groups, a basic foundational understanding of Southeast Asian (SEA) women living in the United States is vital. The purpose of this study was to describ...

Medina, H.; Huang, C-Chi.; Lin, H-Chiao.; Huang, Y-Hsian.; Chen, Y-Ze.; Yen, W-Chun.; Chueh, Y-Lun., 2015: Ultrafast Graphene Growth on Insulators via Metal-Catalyzed Crystallization by a Laser Irradiation Process: From Laser Selection, Thickness Control to Direct Patterned Graphene Utilizing Controlled Layer Segregation Process. Despite the vast progress in chemical vapor deposition (CVD) graphene grown on metals, the transfer process is still a major bottleneck, being not devoid of wrinkles and polymer residues. In this paper, a structure is introduced to directly synthe...

Richart, R.M., 1986: The incidence of cervical and vaginal dysplasia after exposure to DES. JAMA 255(1): 36-37