+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Regulation of transcription of the chromosomal dnaA gene of Escherichia coli

Regulation of transcription of the chromosomal dnaA gene of Escherichia coli

Molecular and General Genetics 205(1): 115-121

By comparative S1 analysis we investigated the in vivo regulation of transcription of the chromosomal dnaA gene coding for a protein essential for the initiation of replication at the chromosomal origin. Inactivation of the protein in dnaA mutants results in derepression, whereas excess DnaA protein (presence of a DnaA overproducing plasmid) leads to repression of dnaA transcription. Both dnaA promoters are subject to autoregulation allowing modulation of transcriptional efficiency by at least 20-fold. Increasing the number of oriC sequences (number of DnaA binding sites) in the cell by introducing oriC plasmids leads to a derepression of transcription. Autoregulation and binding to oriC suggest that the DnaA protein exerts a major role in the regulation of the frequency of initiation at oriC. The efficiency of transcription of the dnaA2 promoter is reduced in the absence of dam methylation, which is involved in the regulation of oriC replication.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 041213736

Download citation: RISBibTeXText

PMID: 3025553

Related references

Transcription termination in the Escherichia coli dnaA gene is not mediated by the internal DnaA box. Journal of Bacteriology 177(7): 1896-1899, 1995

Regulation of expression of the dnaA gene in Escherichia coli: role of the two promoters and the DnaA box. New Biologist 2(6): 574-582, 1990

Titration of DnaA protein by oriC DnaA-boxes increases dnaA gene expression in Escherichia coli. Embo Journal 6(1): 255-258, 1987

Regulation of chromosomal replication by DnaA protein availability in Escherichia coli: Effects of the datA region. Biochimica et Biophysica Acta 1521(1-3): 73-80, 31 October, 2001

A temperature sensitive escherichia coli mutant defective in dna replication dnaa a new gene adjacent to the dnaa gene. Molecular & General Genetics 178(3): 541-554, 1980

Specific binding of DnaA protein to a DnaA box in the guaB gene of Escherichia coli K12. European Journal of Biochemistry 241(2): 411-416, 1996

Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Molecular and General Genetics 209(3): 518-525, 1987

Increased expression of the dnaA gene has no effect on DNA replication in a dnaA+ strain of Escherichia coli. Molecular and General Genetics 192(3): 506-508, 1983

A nucleotide switch in the Escherichia coli DnaA protein initiates chromosomal replication: evidnece from a mutant DnaA protein defective in regulatory ATP hydrolysis in vitro and in vivo. Journal of Biological Chemistry 277(17): 14986-14995, 2002

Requirement of the Escherichia coli dnaA gene function for integrative suppression of dnaA mutations by plasmid R 100-1. Molecular and General Genetics 213(1): 163-165, 1988

Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes and Development 20(15): 2121-2134, 2006