+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Nucleotide sequence comparison of five human pepsinogen A (PGA) genes: evolution of the PGA multigene family

Nucleotide sequence comparison of five human pepsinogen A (PGA) genes: evolution of the PGA multigene family

Genomics 4(3): 232-239

ISSN/ISBN: 0340-6717

PMID: 2714788

DOI: 10.1007/bf00288287

To unravel the genetic basis for the pepsinogen A (PGA) protein polymorphism, we have isolated and characterized a number of PGA genes, distinguishable by polymorphic EcoRI fragments of 12.0, 15.0, and 16.6 kb. Using a HindIII or AvaII polymorphism, we can discriminate between different 15.0 (15.0 and 15.0*) and 12.0 (12.0s and 12.0l) genes, respectively. The coding sequences of a 15.0 and a 16.6 gene were determined, together with considerable stretches of the 5'- and 3'-flanking regions and introns. The genes were demonstrated to encode Pg5 and Pg4, respectively. Because substitutions in codons 43 and 207 appeared to be critical in the determination of the encoded proteins, we sequenced only these regions in the two 12.0 genes and the 15.0* gene. On the basis of these partial sequences, we assume that these genes encode Pg3. In the evolutionary model of the PGA gene cluster presented here, the 12.0 genes arose by an unequal, but homologous crossover. The results of sequence analysis of the second intron of the 12.0s, 12.0l, 15.0, and 16.6 genes suggest that the two 12.0 genes have arisen from two different crossover events.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 041252671

Download citation: RISBibTeXText

Related references

Remarks on the article "H-Y antigenicity of human fibroblasts" by C. Jorgensen and E.J. Eichwald. Human Genetics 81(3): 302-303, 1989

Views on equipment of dental polyclinic for sit-down technic. Discussion on the article by Tor Svensson and Bjöör Wiberg in issue No. 20. Tidning. Sveriges Tandlakarforbund 59(23): 961-962, 1967

H-Y antigenicity of human fibroblasts. Human Genetics 81(2): 182, 1989

Response to remarks by L. Placer on the article: Ring structure of Skofja Loka. Geologija 25(1): 201-204, 1982

Holleman-Wiberg, Lehrbuch der Anorganischen Chemie. Von E. Wiberg. 71–80., völlig umgearbeitete und stark erweiterte Auflage. 3XII/1209 Seiten, 216 Figuren und 37 Strukturbilder in stereoskop. Darst. Walter de Gruyter u. Co., Berlin 1971. Preis: DM 58,–. Archiv der Pharmazie 304(2): 159-160, 1971

Remarks on the article by K. A. Lehmann et al.:CO2 response curves as a measure of opiate-induced respiratory depression. Studies with fentanyl. Der Anaesthesist 33(5): 244-250, 1984

Response of L-929 fibroblasts, human gingival fibroblasts, and human tissue mast cells to various metal cations. Journal of Dental Research 74(8): 1513-1520, 1995

Human wound fibroblasts differ from normal human skin fibroblasts in growth factor response. Pathology Research and Practice 191(7-8): 646, 1995

Differential growth response of normal human diploid fibroblasts and in vitro transformed human fibroblasts in serum-free defined culture medium. International Journal of Cancer 34(5): 645-649, 1984

Some remarks on the growth of human fibroblasts in media containing copper. American Journal of Cancer 22(1): 66-75, 1934

Response to 'Remarks on the article of Packard: Julian Huxley, Uca pugnax and the allometric method'. Journal of Experimental Biology 216(3): 535-536, 2013

Modern feeding of domestic animals and human health. Remarks on the article of H.Vogel in Munch. med. Wochenschr., 1959, 101, 1217. Munch. med. Wochenschr 101: 2286-2287, 1959

Differences in the response of non dividing human diploid fibroblasts to inducers of dna synthesis generated by cycling human diploid fibroblasts and hela cells. Cell Biology International Reports 9(5): 419-428, 1985

Response to the remarks of G. Imhäuser on the article "Modifications of the Voss' hanging hip and its results in the treatment of coxarthrosis". Zeitschrift für Orthopadie und ihre Grenzgebiete 108(1): 149-150, 1970

Remarks on the article 'Cleavage of the Arg-Ile bond in the polypeptide chain of human pancreatic stone protein' by P. Rouimi, J. Bonicel, M. Rovery and A. De Caro. Febs Letters 227(1): 81-84, 1988