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Rho-dependent transcription termination in the tryptophanase operon leader region of Escherichia coli K-12

Stewart, V.; Landick, R.; Yanofsky, C.

Journal of Bacteriology 166(1): 217-223

1986

ISSN/ISBN: 0021-9193
PMID: 2420781
DOI: 10.1128/jb.166.1.217-223.1986
Accession: 041273770
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Recent studies have suggested that expression of the tryptophanase (tna) operon of Escherichia coli is subject to transcription termination-antitermination control (V. Stewart and C. Yanofsky, J. Bacteriol. 164:731-740, 1985). In vivo studies have indicated that the transcribed leader region, tnaL, contains a site or sites of rho-dependent transcription termination (rho is the polypeptide product of the gene rho). We now report direct in vitro evidence that tnaL contains rho-dependent termination sites. In vivo termination appeared to occur at the rho-dependent termination sites identified in vitro. Transcription pausing analyses correlated sites of pausing in tnaL with sites of rho-dependent termination.

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Related References

Bertrand, K.; Squires, C.; Yanofsky, C. 1976: Transcription termination in vivo in the leader region of the tryptophan operon of Escherichia coli Journal of Molecular Biology 103(2): 319-337
Lee, F.; Squires, C.L.; Squires, C.; Yanofsky, C. 1976: Termination of transcription in vitro in the Escherichia coli tryptophan operon leader region Journal of Molecular Biology 103(2): 383-393
Stauffer, G.V.; Zurawski, G.; Yanofsky, C. 1978: Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator Proceedings of the National Academy of Sciences of the United States of America 75(10): 4833-4837
Zurawski, G.; Yanofsky, C. 1980: Escherichia coli tryptophan operon leader mutations, which relieve transcription termination, are cis-dominant to trp leader mutations, which increase transcription termination Journal of Molecular Biology 142(1): 123-129
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