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Secretion of Cellulomonas fimi exoglucanase by Escherichia coli

Secretion of Cellulomonas fimi exoglucanase by Escherichia coli

Gene 44(2-3): 331-336

ISSN/ISBN: 0378-1119

PMID: 3023194

DOI: 10.1016/0378-1119(86)90198-8

A leader sequence of 41 amino acids (aa) has been proposed as the signal sequence for the exoglucanase (Exg) from Cellulomonas fimi. The ability of this 41-aa peptide to function as a leader sequence has been shown here by gene fusion experiments in Escherichia coli. A hybrid leader sequence containing C-terminal 37 aa of the leader peptide and N-terminal 6 aa of beta-galactosidase (beta Gal) directed export of the Exg into the periplasm of E. coli. In contrast, hybrid beta Gal-Exg proteins in which the leader sequence is not present are retained in the cytoplasm.

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Accession: 041317524

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