+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Separate and combined effects of recombinant interleukin-1 alpha and gamma interferon on antibacterial resistance

Separate and combined effects of recombinant interleukin-1 alpha and gamma interferon on antibacterial resistance

Infection and Immunity 57(2): 553-558

Our laboratory has previously reported that administration of murine recombinant interleukin 1 alpha (rIL-1 alpha) substantially enhanced the resistance of mice to Listeria monocytogenes infection. Other investigators have reported that gamma interferon (IFN-gamma) plays a pivotal role in antilisteria resistance. In the present study, we have defined doses of human rIL-1 alpha that enhanced the antilisteria resistance of mice. We then addressed the possibility that combined immunotherapy with rIL-1 alpha and recombinant IFN-gamma (rIFN-gamma) might result in an additive or synergistic enhancement of antibacterial resistance. Simultaneous administration of rIL-1 alpha and rIFN-gamma enhanced antilisteria resistance (at 3 days after infection) to a greater extent than did either cytokine alone, although the results did not imply a synergistic action between the two cytokines. Experiments which examined the effects of the timing of cytokine administration indicated that maximal protection was observed when rIL-1 alpha and rIFN-gamma were administered together concomitantly with the L. monocytogenes challenge. When we compared the separate and combined protective effects of rIL-1 alpha and rIFN-gamma throughout the course of a primary L. monocytogenes infection, we observed an additive effect of the two cytokines only at 3 days after challenge, the time at which the peak bacterial burden occurs in the spleens and livers of infected mice. Histopathological comparisons of livers and spleens from cytokine-treated and control listeria-infected mice verified that cytokine treatment reduced the severity of tissue damage in cytokine-treated listeria-infected mice. In an attempt to provide a potential mechanism for the protective effects of rIL-1 alpha and rIFN-gamma administration, we compared levels of colony-stimulating activity in sera from cytokine-treated and control listeria-infected mice. The highest levels of colony-stimulating activity were detected in sera from control listeria-infected mice; somewhat lower levels were found in sera from listeria-infected mice that received rIL-1 alpha and rIFN-gamma either alone or in combination.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 041332071

Download citation: RISBibTeXText

PMID: 2492263

Related references

Effects of recombinant human interleukin-6 alone and in combination with recombinant interleukin-1 alpha and tumor necrosis factor alpha on antibacterial resistance in mice. Antimicrobial Agents and ChemoTherapy 36(1): 68-70, 1992

Immunomodulatory effects of recombinant alpha interferon gamma interferon and interleukin 2 in vitro. Blut: 229, 1986

Influence of recombinant interferon alpha rifn alpha recombinant interferon gamma rfn gamma and recombinant tumor necrosis factor alpha rtnf alpha on hematopoietic precursor cells from healthy individuals and patients with cml comparison of in vitro sensitivity with the in vitro response to rifn alpha. Immunobiology 175(1-2): 62, 1987

Comparison of the effects of interleukin-1 alpha, interleukin-1 beta and interferon-gamma-inducing factor on the production of interferon-gamma by natural killer. European Journal of Immunology 27(11): 2787-2792, 1997

In vivo sensitivity of hematopoietic precursor cells to recombinant interferon alpha rifn alpha recombinant interferon gamma rifn gamma and recombinant tumor necrosis factor alpha rtnf alpha in normal controls and in patients with cml relationship to the in vivo response. Journal of Interferon Research 7(6): 739, 1987

Regulation of neutrophil migration and superoxide production by recombinant tumor necrosis factors-alpha and -beta: comparison to recombinant interferon-gamma and interleukin-1 alpha. Blood 70(4): 979-984, 1987

Recombinant human interferon-alpha and interferon-beta interleukin-6 but not interferon-gamma induce megakaryocytic differentiation of the acute megakaryoblastic leukaemia blast cells. Pathology Research & Practice 189(6-7): 712, 1993

A phase I trial of recombinant interleukin-2 combined with recombinant interferon-gamma in patients with cancer. Journal of Clinical Oncology 8(7): 1269-1276, 1990

Heterogeneous response of human colon cancer cells to the cytostatic and cytotoxic effects of recombinant human cytokines: interferon-alpha, interferon-gamma, tumor necrosis factor, and interleukin-1. Journal of Biological Response Modifiers 8(2): 206-218, 1989

Effects of recombinant human interleukin 2 il 2 and tumor necrosis factor alpha tnf alpha with or without interferon gamma ifn alpha on human thyroid tissues from patients with graves disease and normal subjects xenografted into nude mice. Clinical Research 39(2): 376A, 1991

Eradication of mouse melanoma by combined treatment with recombinant human interleukin 2 and recombinant murine interferon-gamma. International Journal of Cancer 41(2): 315-322, 1988

Comparison of the central nervous system effects of recombinant interleukin 1 beta il1 recombinant interferon alpha 2 ifn recombinant tumor necrosis factor alpha tnf. Journal of Leukocyte Biology 42(5): 560-561, 1987

Restoration of anti-interleukin-1 depressed natural killer activity by human recombinant interferon alpha or gamma, human recombinant interleukin-2 and indomethacin. International Journal of Immunopharmacology 10(8): 907-911, 1988

Selective impairment of alpha-interferon-mediated natural killer augmentation in Sjögren's syndrome: differential effects of alpha-interferon, gamma-interferon, and interleukin 2 on cytolytic activity. Clinical and Experimental Immunology 70(2): 354-363, 1987

The human IgE germline promoter is regulated by interleukin-4, interleukin-13, interferon-alpha and interferon-gamma via an interferon-gamma-activated site and its flanking regions. European Journal of Biochemistry 240(3): 667-673, 1996