EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Separation of human natural killer cell subpopulations differentially responsive to interferon potentiation



Separation of human natural killer cell subpopulations differentially responsive to interferon potentiation



Journal of Interferon Research 6(4): 361-372



Subsets of human natural killer (NK) cells were identified that differed in the capacity to be activated by interferon (IFN) or the IFN-inducer, polyriboinosinic:polyribocytidylic acid [poly(I):poly(C)]. These subsets, which represented effectors of both spontaneous and antibody-dependent cellular cytotoxicity, were physically separable on the basis of cell buoyant density changes induced by exposure of lymphocytes to hyperosmolar Ficoll-Hypaque solutions or by centrifugation of lymphocytes through hyperosmolar (350 mOs/kg) Percoll gradients. Hyperosmolar conditions per se altered neither cell viability, NK cell cytolytic activity, nor the capacity of NK cells in unseparated lymphocyte preparations to be activated by IFN. IFN-unresponsive NK cells, separated by centrifugation through a 350 mOs/kg Percoll layer of 1.069-1.070 g/cm3 specific density, constituted 20 +/- 4% of all active NK cells identified at the single cell level and, per active NK cell, killed comparably to unstimulated IFN-responsive NK cells in 51Cr release assays. Thus, the IFN-unresponsive phenotype was probably not attributable to NK cells that were in an activated state prior to IFN treatment. Surface marker analysis of active NK cells at the single cell level identified comparable proportions in each subfraction to be of the OKM1+, OKT8+, or OKT11+ phenotypes and few, if any, in either subfraction to be of the OKT3+ phenotype. The human IFN-unresponsive NK cell phenotype, in contrast to the corresponding phenotype in the mouse, was therefore not linked to expression of T-cell-associated membrane differentiation antigens.

(PDF emailed within 0-6 h: $19.90)

Accession: 041333147

Download citation: RISBibTeXText

PMID: 3772177

DOI: 10.1089/jir.1986.6.361



Related references

In vitro potentiation of human natural killer cell activity by a streptococcal preparation, OK-432: interferon and interleukin-2 participation in the stimulation with OK-432. Journal of the National Cancer Institute 69(4): 807-812, 1982

In vitro potentiation of human natural killer cell activity by a streptococcal preparation ok 432 picibanil interferon and interleukin 2 participation in stimulation with ok 432. Journal of the National Cancer Institute 69(4): 807-812, 1982

Potentiation of natural killer cell activity of human lymphocytes in vitro: the participation of interferon in stimulation with Staphylococcus aureus Cowan I bacteria but not with protein A. Immunology 45(4): 687-695, 1982

Potentiation of human natural killer cell cytotoxicity by Salmonella bacteria is an interferon- and interleukin-2-independent process that utilizes CD2 and CD18 structures in the effector phase. Infection and Immunity 59(8): 2767-2773, 1991

Natural killer cell activity in human allogeneic marrow recipients interferon and interleukin 2 natural killer cell interactions. Bone Marrow Transplantation 1(SUPPL 1): 161-162, 1986

Separation of natural killer pre natural killer and epithelial cells from human colonic mucosa demonstration of natural killer cyto toxicity against autologous epithelial targets by a single cell cyto toxic assay. Gastroenterology 80(5 PART 2): 1300, 1981

Human natural killer cell activity is augmented by interferon via recruitment of pre natural killer cells. Scandinavian Journal of Immunology 10(3): 257-266, 1979

Renewal and killer cell recruitment capacity of purified human natural killer nk subpopulations killers binders and free effector cells. FASEB Journal 4(7): A1716, 1990

Studies on the mechanism of natural killer cell mediated cyto toxicity 4 interferon induced inhibition of natural killer target cell susceptibility to lysis is due to a defect in their ability to stimulate release of natural killer cyto toxic factors. 1983

Interferon and natural killer activity in multiple myeloma. Lack of correlation between interferon-induced enhancement of natural killer activity and clinical response to human interferon-alpha. International Journal of Cancer 30(2): 167-172, 1982

Natural killer cell immunodeficiency in patients with chronic myelogenous leukemia iv. interleukin 1 deficiency gamma interferon deficiency and the restorative effects of short term culture in the presence of interleukin 2 on natural killer cytotoxicity natural killer target binding and production of natural killer cytotoxic factor. Natural Immunity and Cell Growth Regulation 10(2): 57-70, 1991

Murine natural killer cell heterogeneity subpopulations of c 57bl 6 splenic natural killer cells detected by nk 1.1 and nk 2.1 antisera. Natural Immunity and Cell Growth Regulation 4(4): 169-177, 1985

Natural killer cell activity in the rat. Analysis of effector cell morphology and effects of interferon on natural killer cell function in the athymic (nude) rat. European Journal of Immunology 12(7): 577-582, 1982

Effect of recombinant alpha-interferon administration on peripheral blood lymphocyte subpopulations and natural killer cell activity in patients with refractory anemia with excess of blasts. Nouvelle Revue Francaise D'hematologie 33(1): 21-24, 1991