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Separation processes in biotechnology. Aqueous two-phase separations



Separation processes in biotechnology. Aqueous two-phase separations



Bioprocess Technology 9: 287-327



Aqueous two-phase systems are useful for separation of a wide range of water-compatible substances (from peptides to cells). The selectivity of the separation normally increases with the size of the partitioned molecules or particles. The partition and separation capacity can be influenced in a number of ways, including electric charge, hydrophobicity, or specific ligand binding. Because of the simpleness in operation and high capacity, aqueous two-phase systems are well suited for large-scale purification of biomaterials such as enzymes and other specific proteins.

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Accession: 041333786

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PMID: 1366882


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