+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Sequence analysis of temperature-sensitive mutations in the Saccharomyces cerevisiae gene CDC28



Sequence analysis of temperature-sensitive mutations in the Saccharomyces cerevisiae gene CDC28



Molecular and Cellular Biology 6(11): 4099-4103



Eleven independently isolated temperature-sensitive mutations in the cell division cycle gene CDC28 were mapped with respect to the DNA sequence of the wild-type gene and then sequenced to determine the precise nature of each mutation. The set yielded six different point mutations, each of which predicts a single amino acid substitution in the CDC28 product. The positions of the mutations did not correlate in any obvious way with observable biological characteristics of the mutant alleles. When the positions of substitutions were collated with a predicted secondary structural analysis of the CDC28 protein kinase, they were found to correlate strongly with probable regions of structural transition.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 041335013

Download citation: RISBibTeXText

PMID: 3540606

DOI: 10.1128/mcb.6.11.4099


Related references

Isolation of a CDC28 homologue from Cryptococcus neoformans that is able to complement cdc28 temperature-sensitive mutants of Saccharomyces cerevisiae. Fems Yeast Research 4(7): 737-744, 2004

Mutations of the CDC28 gene and the radiation sensitivity of Saccharomyces cerevisiae. Yeast 14(2): 133-146, 1998

CDKA and CDKB kinases from Chlamydomonas reinhardtii are able to complement cdc28 temperature-sensitive mutants of Saccharomyces cerevisiae. Protoplasma 232(3-4): 183-191, 2008

Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes. Molecular and Cellular Biology 11(2): 746-753, 1991

Allele-specific suppression of temperature-sensitive mutations of the Saccharomyces cerevisiae RAD52 gene. Current Genetics 29(3): 203-210, 1996

Preliminary identification of a genomic DNA sequence in Sporothrix schenckii homologous to the Saccharomyces cerevisiae cdc28 gene. Abstracts of the General Meeting of the American Society for Microbiology 94: 590, 1994

Analysis of maintenance of redundant genetic structures in the yeast Saccharomyces cerevisiae: Effects of mutations cdc28-srm and srm1. Genetika 31(4): 464-470, 1995

Sequence analysis of a 5.6 kb fragment of chromosome II from Saccharomyces cerevisiae reveals two new open reading frames next to CDC28. Yeast 11(5): 455-458, 1995

Molecular cloning and phenotypic analysis of temperature sensitive and null mutations of RPD3 in Saccharomyces cerevisiae. Abstracts of the General Meeting of the American Society for Microbiology 96: 513, 1996

Temperature sensitive cdc7 mutations of saccharomyces cerevisiae are suppressed by the dbf4 gene which is required for the c 1 s cell cycle transition. Genetics 131(1): 21-29, 1992

Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae. Molecular and Cellular Biology 8(10): 3997-4008, 1988

DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae. Molecular and Cellular Biology 8(2): 978-981, 1988