Section 42
Chapter 41,371

Simultaneous determination of cortisol and cortisone in human plasma by stable-isotope dilution mass spectrometry

Shibasaki, H.; Arai, I.; Furuta, T.; Kasuya, Y.

Journal of Chromatography 576(1): 47-52


ISSN/ISBN: 0021-9673
PMID: 1500456
DOI: 10.1016/0378-4347(92)80173-n
Accession: 041370420

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A method for the simultaneous determination of cortisol and cortisone in human plasma was developed using capillary gas chromatography-mass spectrometry-selected ion monitoring. [2H5]Cortisol and [2H5]cortisone were used as internal standards. Cortisol and cortisone in plasma were determined from the peak-height ratios of the [M-31] fragment ions of the methoxime-trimethylsilyl derivatives of cortisol and [2H5]cortisol (m/z 605 and 610) and of cortisone and [2H5]cortisone (m/z 531 and 536). Sensitivity, specificity, precision, accuracy and reproducibility of the method were demonstrated to be satisfactory for measuring the circulating concentrations of cortisol and cortisone.

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