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Simultaneous determination of tenidap and its stable isotope analog in serum by high-performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry

Avery, M.J.; Mitchell, D.Y.; Falkner, F.C.; Fouda, H.G.

Biological Mass Spectrometry 21(7): 353-357

1992


ISSN/ISBN: 1052-9306
PMID: 1420376
DOI: 10.1002/bms.1200210706
Accession: 041370656

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A specific high-performance liquid chromatographic/atmospheric pressure chemical ionization tandem mass spectrometric assay for the quantitative determination of tenidap and its D3 analog in human serum is described. The assay exhibited a linear dynamic range of 0.1-25 micrograms ml-1. Its precision for the two analytes over the range was 17% or better. The assay validity and its utility to investigate changes in tenidap bioequivalence and pharmacokinetics are presented.

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