EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Slow pulsatile movements of Schwann cells in vitro: a time-lapse cinemicrographic study



Slow pulsatile movements of Schwann cells in vitro: a time-lapse cinemicrographic study



Cell Motility and the Cytoskeleton 6(6): 595-603



Slow pulsatile movements of Schwann cells in vitro were studied quantitatively by using time-lapse cinemicrography. Schwann cells from peripheral nerves of 3-day-old rats were cultured in serum-free medium. Most Schwann cells showed intermittent episodes of pulsatile movement; each episode consisted of one or several contractile pulses. About half of the episodes consisted of a single pulse, and episodes with more than four pulses were rare. The average episode of activity lasted 2.6 min, while the average duration of a single pulse was 1.5 min. The mean quiescent interval between episodes of activity was 3.7 min. Some cells showed no pulsatile activity. Active cells averaged 6.6 episodes/h. The fraction of time which a Schwann cell spent in pulsatile activity varied widely, with an average of 28%. Behavior of Schwann cells in HEPES-buffered Hanks saline was generally similar to that in the complete medium. Raising K+ to 40 mM or Ca++ to 10 mM did not markedly affect the time course of the pulsatile motility, although the contractions were more vigorous in the high Ca++. Pulsatile movement was reversibly inhibited by cytochalasin B and appeared to be potentiated by drugs that disrupt microtubules.

(PDF same-day service: $19.90)

Accession: 041381153

Download citation: RISBibTeXText

PMID: 3802219

DOI: 10.1002/cm.970060608



Related references

A time lapse cinemicrographic study of giant cells in populations of sv 40 transformed cells. In Vitro Cellular & Developmental Biology 26(9): 846-848, 1990

A time lapse cinemicrographic study of giant cells in populations of simian virus 40 transformed cells. In Vitro Cellular & Developmental Biology 26(9): 846-848, 1990

Effects of subacute concentrations of acrylamide on the morphology of cultured mouse neuroblastoma N1E115 cells: a time-lapse cinemicrographic study. Alternatives to laboratory animals: ATLA 12(1): 33-41, 1984

Time lapse cinemicrographic studies on behavior of Coccidioides immitis in vitro. Proceedings of the Second Symposium on Coccidioidomycosis, 8-10 December, 1965 409-414, 1967

Time-lapse cinemicrographic studies of x-irradiated HeLa S3 cells. II. Cell fusion. Biophysical Journal 9(9): 1131-1143, 1969

Interactions of elastin fibers with fibroblasts a time-lapse cinemicrographic study. Pathologie-Biologie 46(7): 507-516, 1998

Effect of a phenoxy herbicide on mammalian cell cultures. A time-lapse cinemicrographic study. Acta Pharmacologica et Toxicologica 28(1): 57-57, 1970

Time-lapse cinemicrographic observations of heated G1-phase Chinese hamster ovary cells II. Cell death, fusion, and multipolar divisions. Radiation Research 92(2): 332-342, 1982

Time lapse cinemicrographic studies of x-irradiated HeLa S3 cells. I. Cell progression and cell disintegration. Biophysical Journal 9(4): 607-633, 1969

A simplified time-lapse cinemicrographic unit. Journal of Microscopy 89(1): 151-157, 1969

Design of a phase contrast time-lapse cinemicrographic unit. Journal of the Biological Photographic Association 34(2): 53-82, 1966

Radiation-induced mammalian cell death: lapse-time cinemicrographic observations. Experimental Cell Research 43(2): 413-423, 1966

Time lapse cinemicrographic studies of epithelial and fibroblast behavior on grooved substrate. Journal of Cell Biology 101(5 PART 2): 252A, 1985

Time-lapse cinemicrographic analysis of the inhibition of ontogeny in the alga Scenedemus quadricauda by 6-azauridine. Biochemie und Physiologie der Pflanzen73(3): 279-283, 1978