EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Slow pulsatile movements of Schwann cells in vitro: a time-lapse cinemicrographic study






Cell Motility and the Cytoskeleton 6(6): 595-603

Slow pulsatile movements of Schwann cells in vitro: a time-lapse cinemicrographic study

Slow pulsatile movements of Schwann cells in vitro were studied quantitatively by using time-lapse cinemicrography. Schwann cells from peripheral nerves of 3-day-old rats were cultured in serum-free medium. Most Schwann cells showed intermittent episodes of pulsatile movement; each episode consisted of one or several contractile pulses. About half of the episodes consisted of a single pulse, and episodes with more than four pulses were rare. The average episode of activity lasted 2.6 min, while the average duration of a single pulse was 1.5 min. The mean quiescent interval between episodes of activity was 3.7 min. Some cells showed no pulsatile activity. Active cells averaged 6.6 episodes/h. The fraction of time which a Schwann cell spent in pulsatile activity varied widely, with an average of 28%. Behavior of Schwann cells in HEPES-buffered Hanks saline was generally similar to that in the complete medium. Raising K+ to 40 mM or Ca++ to 10 mM did not markedly affect the time course of the pulsatile motility, although the contractions were more vigorous in the high Ca++. Pulsatile movement was reversibly inhibited by cytochalasin B and appeared to be potentiated by drugs that disrupt microtubules.


Accession: 041381153

PMID: 3802219

DOI: 10.1002/cm.970060608



Related references

Lehman J.M., 1990: A time lapse cinemicrographic study of giant cells in populations of sv 40 transformed cells. In Vitro Cellular & Developmental Biology 26(9): 846-848

Lehman, J.M., 1990: A time lapse cinemicrographic study of giant cells in populations of simian virus 40 transformed cells. In Vitro Cellular & Developmental Biology 26(9): 846-848

Walum, E.P.terson, A., 1984: Effects of subacute concentrations of acrylamide on the morphology of cultured mouse neuroblastoma N1E115 cells: a time-lapse cinemicrographic study. Alternatives to laboratory animals: ATLA 12(1): 33-41

Brosbe, A.A.; Kietzman, J.N.; Snellen, J.E., 1967: Time lapse cinemicrographic studies on behavior of Coccidioides immitis in vitro. The behavior of tissue and cultured spherules of C. immitis was recorded by time lapse cinemicrography. The protoplasmic activity was predominantly that of vacuolization. In the system employed, activity diminished greatly after 36 hr. Cleavage wa...

Hurwitz, C.; Tolmach, L.J., 1969: Time-lapse cinemicrographic studies of x-irradiated HeLa S3 cells. II. Cell fusion. Analysis of time-lapse cinemicrographs of X-irradiated HeLa S3 cells has shown that the incidence of cell fusion was increased from 0.9% (following 1267 divisions) in control cells to an average of 22% (following 655 divisions) in cells irradiated...

Groult, V.; Hornebeck, W.; Robert, L.; Pouchelet, M.; Jacob, M.P., 1998: Interactions of elastin fibers with fibroblasts a time-lapse cinemicrographic study. Adhesion of cells to the extracellular matrix is mediated by structural glycoproteins such as fibronectin and laminin, and also elastonectin, whose role is to ensure binding of elastin fibers to cells. Interactions between elastin fibers and human...

Kolberg, J.; Helgeland, K.; Jonsen, J.; Tjeltveit, O., 1970: Effect of a phenoxy herbicide on mammalian cell cultures. A time-lapse cinemicrographic study. Acta Pharmacologica et Toxicologica 28(1): 57-57

Zielke-Temme, B.; Hopwood, L., 1982: Time-lapse cinemicrographic observations of heated G1-phase Chinese hamster ovary cells. I. Division probabilities and generation times. Chinese hamster ovary [CHO] cells were heated in the G1 phase of the cell cycle following synchronization by mitotic selection. The following temperatures and durations were used: 41.5.degree. C for 200 min, 43.5.degree. C for 50 min, and 45.5.deg...

Zielke-Temme, B.; Hopwood, L., 1982: Time-lapse cinemicrographic observations of heated G1-phase Chinese hamster ovary cells II. Cell death, fusion, and multipolar divisions. Radiation Research 92(2): 332-342

Hurwitz, C.; Tolmach, L.J., 1969: Time lapse cinemicrographic studies of x-irradiated HeLa S3 cells. I. Cell progression and cell disintegration. Time-lapse cinemicrographs of synchronous HeLa S3 cells irradiated with 220 kv X-rays at various stages of interphase provided data for constructing pedigrees, measuring the duration of both generation cycles and mitoses, and scoring events associ...