+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Specific effect of anti-transferrin antibodies on natural killer cells directed against tumor cells. Evidence for the transferrin receptor being one of the target structures recognized by NK cells



Specific effect of anti-transferrin antibodies on natural killer cells directed against tumor cells. Evidence for the transferrin receptor being one of the target structures recognized by NK cells



Journal of Immunology 134(2): 1286-1291



Treatment of PBL or Percoll-isolated LGL with anti-transferrin antibodies plus complement reduced their natural killing activity against K-562 cells between 30 and 70%. The same antibodies inhibited natural cytotoxicity when added directly to the assay. Similar depletion or inhibition of NK cytotoxicity was observed when using HeLa cells as targets. The decrease or inhibition by transferrin antibodies was less marked when IFN-treated PBL or LGL as effector cells were used. The inhibition of anti-transferrin antibodies seems to be located at the level of the effector cell population. When PBL but not target K-562 cells were pretreated with anti-transferrin antibodies and were washed before use in the assay, cytotoxicity was decreased by 50%. In addition, about 80% of the LGL positively selected on anti-transferrin plates stained with Leu-11. Furthermore, no reduction by anti-transferrin antibodies plus complement treatment of PBL or LGL, or inhibition by antibodies alone, was observed when the cells were tested against HSV-1-infected cells. Membrane extracts from LGL inhibited NK cytotoxicity against K-562 or HeLa cells. Moreover, the inhibitory component of this extract was removed by anti-transferrin IgG but not by control IgG. These results are in agreement with the recent hypothesis that NK cells recognize the transferrin receptor in tumor target cells, because both the transferrin receptor and anti-transferrin antibodies may share a similar structure that interacts with the NK cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041406090

Download citation: RISBibTeXText

PMID: 2981268


Related references

Studies on the mechanism of specificity of human natural killer cells for tumor cells correlation between target cell transferrin receptor expression and competitive activity. Cellular Immunology 96(2): 255-266, 1985

Target structure for natural killer cells: evidence against a unique role for transferrin receptor. European Journal of Immunology 14(4): 340-344, 1984

Transferrin receptors on tumor and bone marrow cells lack of involvement as target structure for natural killer cells. Klinische Wochenschrift 64(21): 1119-1123, 1986

Demonstration of the specific binding of bovine transferrin to the human transferrin receptor in K562 cells: evidence for interspecies transferrin internalization. Journal of Cellular Physiology 128(1): 1-8, 1986

Receptor for transferrin may be a target structure for natural killer cells. Proceedings of the National Academy of Sciences of the United States of America 80(3): 835-839, 1983

Studies on the mechanism of natural killer cell mediated cyto toxicity 1. release of cyto toxic factors specific for natural killer sensitive target cells during co culture of natural killer effector cells with natural killer target cells. Journal of Immunology 129(1): 433-439, 1982

The low affinity 40,000 Fc gamma receptor and the transferrin receptor can be alternative or simultaneous target structures on cells sensitive for natural killing. Journal of Immunology 136(12): 4714-4720, 1986

The low affinity 40 kilodalton fcyr and the transferrin receptor can be alternative or simultaneous target structures on cells sensitive for natural killing. Uicc (Union Internationale Contre Le Cancer, International Union Against Cancer) 14th International Cancer Congress, Budapest, Hungary, Aug 21-27, Abstracts, Lectures, Symposia And Free Communications, Vols 1, 2, 3, Late Abstracts, And Register Xvi+479p (Vol 1); Xvi+298p (Vol 2); Xvi+531p (Vol 3); 15p (Late Abstracts); 40p (Register) S Karger Ag: Basel, Switzerland; New York, N Y , Usa; Akademiai Kiado: Budapest, Hungary Paper 925, 1986

The low affinity 40 kilodalton fc gamma r and the transferrin receptor can be alternative or simultaneous target structures on cells sensitive for natural killing. Federation Proceedings 45(3): 384, 1986

Effects of different transferrin forms on transferrin receptor expression, iron uptake, and cellular proliferation of human leukemic HL60 cells. Mechanisms responsible for the specific cytotoxicity of transferrin-gallium. Journal of Clinical Investigation 78(6): 1538-1546, 1986

Effects of monoclonal anti-transferrin receptor antibodies on in vitro growth of human solid tumor cells. Cancer Research 47(8): 2040-2044, 1987

Recognition of k 562 by natural killer cells does not seem to involve the transferrin receptor. Scandinavian Journal of Immunology 22(4): 448, 1985

The ability of anti-transferrin receptor antibodies to inhibit iron uptake in tumor cells appears to be related to the initial cell surface density of the receptor. FASEB Journal 9(4): A1045, 1995

Nature of the target structures recognized by natural killer cells. Eksperimental'naya Onkologiya 13(1): 8-14, 68, 1991

Use of anti-HLA antibodies to mask major histocompatibility complex gene products on tumor cells can enhance susceptibility of these cells to lysis by natural killer cells. Journal of Clinical Investigation 83(1): 278-287, 1989