EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of new peptide immunoassays versus other immunoassays and agglutination tests for detection of HIV antibody in African sera


European Journal of Clinical Microbiology & Infectious Diseases 10(8): 636-640
Specificity of new peptide immunoassays versus other immunoassays and agglutination tests for detection of HIV antibody in African sera
The specificity of three HIV EIAs using synthetic peptides was evaluated and compared with that of four commercial EIAs using recombinant proteins or whole virus lysates of HIV-1 or of HIV-1 and HIV-2, and of two tests based on agglutination of sensitized particles. A total of 734 West African sera were screened. The best results were obtained with tests using recombinant proteins. HIV tests using synthetic peptides able to distinguish between HIV-1 and HIV-2 antibodies showed comparable specificity, but some false negative results represent a major handicap of these peptide EIAs in screening for HIV antibodies.


Accession: 041408148

PMID: 1660808

DOI: 10.1007/bf01975815



Related references

Comparison of immunofluorescence, particle agglutination, and enzyme immunoassays for detection of human T-cell leukemia virus type I antibody in African sera. Journal of Clinical Microbiology 28(9): 1988-1993, 1990

Comparison of 10 enzyme immunoassays for detection of antibody to human immunodeficiency virus type 2 in West African sera. Journal of Clinical Microbiology 26(5): 1000-1004, 1988

Comparison of enzyme immunoassays and an immunofluorescence test for detection of antibody to human immunodeficiency virus in African sera. European Journal of Clinical Microbiology 6(2): 132-135, 1987

Use of monoclonal antibody to increase the sensitivity and specificity of precipitating immunoassays and cell surface binding immunoassays. Methods in Enzymology 121: 702-717, 1986

Characterization of Treponema pallidum particle agglutination assay-negative sera following screening by treponemal total antibody enzyme immunoassays. Clinical and Vaccine Immunology 17(11): 1718-1722, 2011

Hiv 1 antibody testing using recombinant antigens with latex and enzyme immunoassays of african sera. Abstracts of the Annual Meeting of the American Society for Microbiology 90: 434, 1990

Comparison of two enzyme immunoassays and two latex agglutination assays for detection of cytomegalovirus antibody. Diagnostic Microbiology & Infectious Disease. 20(2): 109-112, 1994

Peptide based enzyme-linked immunoassays for detection of anti-HSV-2 IgG in human sera. Journal of Virological Methods 87(1-2): 63-70, 2000

Peptide based enzyme-linked immunoassays for detection of anti-HSV-2 IgG in human sera. Journal of Virological Methods 87(1-2): 63-70, June, 2000

Evaluation of two enzyme immunoassays for detection of human immunodeficiency virus antigen in African and European sera. European Journal of Clinical Microbiology & Infectious Diseases 8(10): 892-895, 1989