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Specificity of the Crithidia luciliae method for detecting anti-DNA antibodies. Effect of absorption for lipoproteins

, : Specificity of the Crithidia luciliae method for detecting anti-DNA antibodies. Effect of absorption for lipoproteins. Immunological Investigations 14(3): 199-210

Using the immunofluorescent (IF) assay with Crithidia luciliae smears, anti-native (n) DNA antibodies were detected in the sera of 12 of 20 systemic lupus erythematosus (SLE) patients, in 1 of 6 mixed connective tissue disease cases, in 2 of 38 patients with systemic sclerosis but in none of the sera from 96 normal subjects. All anti-nDNA antibodies were associated with antinuclear antibodies (ANA). However, occasionally sera were encountered in routine screening which appear to be positive for anti-DNA antibodies but negative for ANA. Studies of such sera indicate that this is a nonspecific reaction which can be abolished by treating sera with dextran sulfate or heparin. Treatment of SLE sera with these agents had no effect on their anti-nDNA antibody activity. Absorption of sera with Aerosil eliminated the false positive reactions with C. luciliae; however, this treatment also removed immunoglobulins, ANA and anti-nDNA antibodies. Evidence is reviewed which points to a role of complexes of low density lipoprotein and IgG in the nonspecific binding reactions with C. luciliae which is seen as false positive reactions for anti-nDNA antibodies.

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Accession: 041408259

PMID: 2995252

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Related references

Kumar, V.; Krasny, S.; Beutner, E.H., 1985: Specificity of Crithidia luciliae method for detecting anti-DNA antibodies. Effect of absorption for lipoproteins. Journal of Rheumatology 12(2): 382-384

Steinmetz, S.E.; Deng, J.S.; Rubin, R.L.; Sontheimer, R.D.; Gilliam, J.N., 1984: Reevaluation of specificity of Crithidia luciliae kinetoplast as a substrate for detecting antibodies to double-stranded deoxyribonucleic acid. Until now, the kinetoplast of the hemoflagellate Crithidia luciliae has been believed to be a specific indirect immunofluorescence substrate for detecting antibodies to double-stranded deoxyribonucleic acid (DNA). We have recently identified a pat...

Anonymous, 1984: Superiority of crithidia luciliae immuno peroxidase vs crithidia luciliae incident and transmitted immuno fluorescence methods for detection of anti double stranded dna antibodies

Hu, D.W., 1983: Determination of anti-double stranded DNA antibodies by the Crithidia Luciliae immunofluorescence method and its clinical significance. Zhonghua Nei Ke Za Zhi 22(6): 336-338

Pigrau, C.; Martínez-Vázquez, J.M.; Bacardi, R.; Pahissa, A.; Pastor, M.; Guardia, J., 1984: Clinical significance of anti-native DNA antibodies in systemic lupus erythematosus by the Crithidia luciliae method. Medicina Clinica 83(6): 227-231

Herbert, K.E.; Jeffery, E.; Scott, D.L., 1990: Effect of fibronectin on the Crithidia luciliae test for anti-double-stranded DNA antibodies. The various tests for anti-double-stranded DNA antibodies do not always agree. Plasma fibronectin specifically binds DNA, is a component of immune complexes, and shows variations in concentration with disease activity in systemic lupus erythematos...

Mahakittikun, V.; Wongkamchai, S.; Sittapairochana, C.; Suthipinitharm, P.; Chaihirankarn, S.; Udompuntirak, S., 1997: Indirect immunoperoxidase staining of Crithidia luciliae for detecting anti-dsDNA: comparison with other serodiagnostic tests. The indirect immunoperoxidase (IIP) method was modified and improved for detecting anti-dsDNA. Using fetal calf serum to block the non-specific antibodies and staining with chromatic substrate solution at pH 6.2, the performance time is substantia...

Konstantinov, K.N.; Russanova, V.R., 1987: Evidence for absence of histones in the Crithidia luciliae kinetoplast: a study with anti-H2A and monoclonal anti-H3 antibodies. Recently, the specificity of the Crithidia luciliae immunofluorescent assay for the detection of antibodies to double-stranded deoxyribonucleic acid (dsDNA) has been questioned. It has been proposed that the kinetoplast of this trypanosome-like or...

André, C.; Villeminot, S.; Bourhala, S., 1982: Immunofluorescence on Crithidia luciliae in the detection of anti-native DNA antibodies. Antibodies to double-stranded (native) DNA were detected in 886 sera by indirect immunofluorescence (IF) using Crithidia luciliae (CL) as substrate. Comparison with 208 sera tested by Farr's method showed that the IF-on-CL method was more sen...

Pena Sagredo, J.L.; Orte Martinez, F.J.; Sanchez Andrada, S.; Sampedro Alvarez, J.; Novo Fernandez, M.J.; Rodriguez Valverde, V., 1982: Anti-DNA determination using Crithidia luciliae. Specificity and clinical usefulness in systemic lupus erythematosus. The authors determined the existence of native anti-DNA antibodies by the crithidia lucilae (anti-DNA-CL) test in 44 patients suffering from systemic lupus erythematosus (SLE), 48 with rheumatoid arthritis (RA), 12 with scleroderma, 8 with polymyo...