EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Specificity of the glucan-binding lectin of Streptococcus cricetus


, : Specificity of the glucan-binding lectin of Streptococcus cricetus. Infection and Immunity 56(8): 1864-1872

The specificity of the glucan-binding lectin (GBL) of Streptococcus cricetus AHT was determined. Examination of the kinetics of aggregation of cell suspensions with glucans containing various percentages of alpha-1,6, alpha-1,4, alpha-1,3, and alpha-1,2 anomeric linkages revealed that only glucans with at least 80% alpha-1,6 linkages promoted strong aggregation. Moreover, only linear glucans with molecular weights greater than 5 X 10(5) were capable of causing rapid aggregation of the bacteria. The lectin was observed to be present on S. cricetus strains, on Streptococcus sobrinus, and on several Streptococcus mutants strains. Preincubation of suspensions of S. cricetus AHT with glucan T10 (molecular weight of 10,000) before the addition of high-molecular-weight glucan resulted in competitive inhibition in a concentration-dependent manner. Inhibition was achieved also with isomaltopentaose, isomaltohexaose, and isomaltooctaose, but at higher concentrations than glucan T10. In contrast, no inhibition was observed with maltoheptaose, providing additional evidence for the specificity of GBL. Treatment of suspensions of S. cricetus AHT with trypsin before and after aggregation with high-molecular-weight glucan revealed a substantial level of protection of GBL when in a bound state. Collectively, these results indicated that GBL has an absolute affinity for glucans rich in alpha-1,6 linkages and possesses an active site which recognizes internal sequences and accommodates isomaltosaccharides of at least nine residues. This unusual specificity may contribute to the colonization of S. cricetus, S. sobrinus, and S. mutans in glucan-containing plaque in the oral cavity.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 041408308

PMID: 3397177

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Perry, Raquel, L.; Barratt, Christopher, L. R.; Warren, Michael, A.; Cooke, Ian, D., 1997: Elevating intracellular calcium levels in human sperm using an internal calcium ATPase inhibitor, 2,5-di(tert-butyl) hydroquinone (TBQ), initiates capacitation and the acrosome reaction but only in the presence of extracellular calcium. The aim of this study was to investigate the effect of an internal calcium ATPase inhibitor, TBQ, on human sperm capacitation and the acrosome reaction during incubation in a calcium-depleted media. Sperm were isolated into and incubated for up to...

Lal, S. P.; Nita Mathur, 1989: Preservation of culture of fungi and Actinomycetes under liquid paraffin. Details are given of the survival of 32 cultures of Actinomycetes, 140 of Ascomycetes and 67 of Deuteromycetes after 6 yr of storage under liquid paraffin.

Ghods, A.J.; Nasrollahzadeh, D.; Argani, H., 2003: Risk factors for noncompliance to immunosuppressive medications in renal transplant recipients. Transplantation Proceedings 35(7): 2609-2611

Drangova, Maria; Pelc, Norbert J., 1996: Artifacts and signal loss due to flow in the presence of B-0 inhomogeneity. An in vitro study was performed to investigate the effects of B-o inhomogeneity on magnetic resonance images of flow. Controlled inhomogeneity gradients (G-i) were applied and the magnitude of the artifacts produced was quantified for different ec...

Daniau, P.; Gueguen, F.; Leca, J. L.; Prove, P., 1980: Rimidine plus, a special fungicide presenting new possibilities for the treatment of growing cereals. The compound (1.6% fenarimol, 8% carbendazim and 64% maneb), sprayed at 2.5 kg/ha, was effective against Cercosporella [Pseudocercosporella] herpotrichoides and Fusarium nivale [Micronectriella nivalis] on wheat and barley, Erysiphe graminis and R...

Nagao M.; Kamo H.; Akiguchi I.; Kimura J., 1992: Soybean agglutinin binds commonly to a subpopulation of small diameter neurons in dorsal root ganglion vascular endothelium and microglia in human spinal cord. Soybean agglutinin (SBA) was used to identify the location of N-acetylgalactosaminyl glyconjugates in human dorsal root ganglia (DRG) and spinal cord. SBA bound to a subpopulation of small-diameter neurons in DRG and their central projections. It...

Mallov, Samuel; Mckibbin, John M.; Robb, Jane Sands, 1952: Essential lipid components of beef heart muscle and conducting tissue. Jour Pharmacol And Exptl Therap 106(4): 405-406

Soler I.Vazquez C.; Nunez De Murga M.; Nunez De Murga J.; Martinez Lopez F.; Nunez Cachaza A., 1990: Comparative analysis of morphometric results obtained by different computer assisted methods in melanoides tuberculata mueller gastropoda thiaridae. Iberus 9(1-2): 489-492

Hovenden,M.J.; Williams,A.L.; Pedersen,J.K.; Schoor,J.K.vander; Wills,K.E., 2008: Elevated CO2 and warming impacts on flowering phenology in a southern Australian grassland are related to flowering time but not growth form, origin or longevity. Flowering is a critical stage in plant life cycles, and changes in phenology might alter processes at the species, community and ecosystem levels. Therefore, likely flowering-time responses to global-change drivers are needed for predictions of gl...

Gold, R. M.; Sumprer, G.; Ueberacher, H. M.; Kapatos, G., 1975: Hypothalamic hyperphagia despite imposed diurnal or nocturnal feeding and drinking rhythms. Rats normally feed most at night but when made hyperphagic by damage to the hypothalamus increases in eating occur mainly during the day, suggesting that a disruption in circadian rhythms may mediate overeating. Female Carworth CFE rats weighing 2...