EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Spectral analysis of high resolution direct-derivative melting curves of DNA for instantaneous and total base composition


, : Spectral analysis of high resolution direct-derivative melting curves of DNA for instantaneous and total base composition. Biochimica et Biophysica Acta 518(2): 233-246

Derivative melting profiles of DNA have been obtained directly by recording the difference in absorbance between two identical solutions maintained at a small constant temperature differential. This deltaA is monitored continuously with increasing temperature in a ratio recording spectrophotometer. Resolution of complex hyperfine structure in the profiles of small homogeneous viral DNAs appears to be significantly better than has been produced by various numerical methods of differentiation. In addition, a spectral method has been modified that permits easy analysis for DNA base composition from the ratio of derivative melting curves obtained at 282 and 260 nm. Eight bacterial and three vertebrate DNAs have been analyzed for total base composition from the product of the instantaneous base composition at small temperature intervals (0.05 degrees C) throughout the entire melting region and the integrated area of the 282 nm profile. The results are in excellent agreement with values determined by traditional methods.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 041408639

PMID: 418812

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Miller, L.H.; Katz, S.I.; Moshell, A.N., 1989: NIH centennial: the influence of NIH on dermatology research training. Journal of Investigative Dermatology 92(4 Suppl): 177s-178s

Rafols, D.; Steiner, J.; Rafols, J.A.; Petrov, T., 2004: Intracellular coexpression of endothelin-1 and inducible nitric oxide synthase underlies hypoperfusion after traumatic brain injury in the rat. We used Marmarou's rat model of traumatic brain injury to demonstrate colocalization of mRNAs for endothelin-1 (ET-1, a powerful vasoconstrictor) and inducible nitric oxide synthase (iNOS, generator of NO, a vasodilator) in individual cells t...

Karlen, Bo; Agren, Allan, 1960: Alkaline hydrolysis of some local anaesthetic esters. The alkaline hydrolysis of the following local anesthetic esters was studied benzocaine, butyl benzocaine, tetracaine, procaine, panthesine and o-hydroxyprocaine. The velocity constants are determined photometrically in H2O solution at 25.0, 35.0...

Hardisty, R.M.; Hutton, R.A., 1966: Platelet aggregation and the availability of platelet factor 3. By simultaneous measurement of changes in optical density and Stypven time of platelet-rich plasma (PRP), the effect of platelet aggregation on the availability of platelet factor 3 was studied. Aggregation by adenosine diphosphate (ADP), adrenali...

Watteau, Francoise; Berthelin, Jacques, 1992: Presence of trihydroxamate siderophores in the different organic matter fractions of calcimagnesic and acid soils. Amounts of biologically active trihydroxamate siderophores were measured in the different fractions of the organic matter of a calcimagnesic soil and of an acidic soil, on which the same plant, Mercurialis perennis, was growing. The acid soil cont...

Kempner, Es; Miller, Jh, 1972: The molecular biology of Euglena gracilis. VII. Inorganic requirements ofr a minimal culture medium. Journal of protozoology: 19 (2) 343-346

Bass, M.; Gardelle, O.; Grest, P.; Bernasconi, C., 2004: Localized histiocytic sarcoma in a dog: an uncommon diagnosis in forelimb lameness. The clinical, ultrasonographic, arthrographic, and histopathological aspects of a localized histiocytic sarcoma in the tendon sheath of the biceps brachii muscle in a Flat-Coated Retriever were described [Switzerland; date not given]. An 8-year-ol...

Sackett, W. W., Jr., 1956: Use of solid foods in early infancy. The author claims from experience with more than 600 babies that they " get along very well " when solid foods are given soon after birth. His idea in writing is "to convince the mothers of the wisdom of making the babies conform to...

Rothenburger W., 1973: How to define processes for the production of vegetables from sowing to harvesting at various times of the year and by various cultivation methods. Acta Horticulturae (Wageningen) 27: 23-43

Osman, Farhat; Jarrous, Nayef; Ben Asouli, Yitzhak; Kaempfer, Raymond, 1999: A novel 3-UTR element in the TNF-alpha gene activates PKR to enhance mRNA splicing without impeding translation. Genes & Development 13(24): 3280-3293, Dec 15