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Spectrophotometric determination of oleanolic acid and saponins from quinoa (Chenopodium quinoa Willd, Kancolla variety)


Spectrophotometric determination of oleanolic acid and saponins from quinoa (Chenopodium quinoa Willd, Kancolla variety)



Archivos Latinoamericanos de Nutricion 38(1): 113-131



ISSN/ISBN: 0004-0622

PMID: 3256282

Saponins were extracted from quinoa (Chenopodium quinoa Willd, Kancolla variety) by refluxing with a methanol-water (4:1) mixture. Once the methanol was evaporated, the remaining residue was treated following Honerlagen and Tretter's method with only slight modifications. The extract was then hydrolyzed with 12N sulfuric acid in a 1:1 dioxane-water system at 110 degrees C for 1.5 hr. The sapogenins were extracted with chloroform, concentrated and some microliters (equivalent to 121 mg of quinoa) were spotted, against an oleanolic acid standard, on a silicagel g plate and developed with a chloroform-acetone-benzene (80:20:10; v/v) mixture. The spots were located by iodine vapor, and the band whose Rf was similar to that of the oleanolic acid, was scraped into a glass column, eluted with chloroform, dried out, dissolved in 1 ml of glacial acetic acid, treated with 4 ml of (1:1; v/v) sulfuric acid:glacial acetic acid mixture, heated in a water bath at 60 degrees C for 25 minutes, cooled and taken to the spectrophotometer where it was read at a wave length of 527 nm against a reagent blank. Under the same conditions, the oleanolic acid employed as a standard showed a linearity in the range of 60 to 480 micrograms. The oleanolic acid percentage has been determined (0.269 +/- 0.025) in quinoa, and the content of saponins estimated using a conversion factor found by gas chromatography and expressed in the following relationship: % Saponin = (8.5204) x (% oleanolic acid) The sapogenin extract obtained - analyzed by this method - showed an error of 10.7% in relation to its gas chromatography determination.

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Accession: 041409330

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