Section 42
Chapter 41,450

Structure and function of the repressor of bacteriophage lambda. III. Molecular cloning of the high-affinity mutant ci gene of lambda and studies of the properties of the clones

Das, T.; Mandal, N.C.

Molecular and General Genetics Mgg 204(3): 540-542


ISSN/ISBN: 0026-8925
PMID: 3020384
DOI: 10.1007/bf00331037
Accession: 041449408

Download citation:  

The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively, the level of repressor in a monolysogen of lambda cIha. Results of the study of certain properties of the bacteria carrying these plasmids suggest that the ha repressor also has a higher affinity for the virulent mutant operators as well as the prm promoter of lambda.

PDF emailed within 0-6 h: $19.90