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Structure and function of the repressor of bacteriophage lambda. III. Molecular cloning of the high-affinity mutant ci gene of lambda and studies of the properties of the clones

Das, T.; Mandal, N.C.

Molecular and General Genetics Mgg 204(3): 540-542

1986

ISSN/ISBN: 0026-8925
PMID: 3020384
DOI: 10.1007/bf00331037
Accession: 041449408
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The high-affinity mutant cI gene of lambda cIha (Nag et al. 1984) was cloned in the multicopy plasmid pBR322. In the resulting plasmid, pMD 102, a lacUV5 promoter was inserted giving the lacUV5-cIha fusion plasmid pMD 205. Bacteria carrying pMD 102 and pMD 205 contain 2.5 and 15 times, respectively, the level of repressor in a monolysogen of lambda cIha. Results of the study of certain properties of the bacteria carrying these plasmids suggest that the ha repressor also has a higher affinity for the virulent mutant operators as well as the prm promoter of lambda.

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Related References

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Wulff, D.L. 1976: Lambda cin-1, a new mutation which enhances lysogenization by bacteriophage lambda, and the genetic structure of the lambda cy region Genetics 82(3): 401-416
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