EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Structure and protein environment of the retinal chromophore in light- and dark-adapted bacteriorhodopsin studied by solid-state NMR


Structure and protein environment of the retinal chromophore in light- and dark-adapted bacteriorhodopsin studied by solid-state NMR



Biochemistry 28(22): 8897-8904



ISSN/ISBN: 0006-2960

PMID: 2605231

DOI: 10.1021/bi00448a032

Our previous solid-state 13C NMR studies on bR have been directed at characterizing the structure and protein environment of the retinal chromophore in bR568 and bR548, the two components of the dark-adapted protein. In this paper, we extend these studies by presenting solid-state NMR spectra of light-adapted bR (bR568) and examining in more detail the chemical shift anisotropy of the retinal resonances near the ionone ring and Schiff base. Magic angle spinning (MAS) 13C NMR spectra were obtained of bR568, regenerated with retinal specifically 13C labeled at positions 12-15, which allowed assignment of the resonances observed in the dark-adapted bR spectrum. Of particular interest are the assignments of the 13C-13 and 13C-15 resonances. The 13C-15 chemical resonance for bR568 (160.0 ppm) is upfield of the 13C-15 resonance for bR548 (163.3 ppm). This difference is attributed to a weaker interaction between the Schiff base and its associated counterion in bR568. The 13C-13 chemical shift for bR568 (164.8 ppm) is close to that of the all-trans-retinal protonated Schiff base (PSB) model compound (approximately 162 ppm), while the 13C-13 resonance for bR548 (168.7 ppm) is approximately 7 ppm downfield of that of the 13-cis PSB model compound. The difference in the 13C-13 chemical shift between bR568 and bR548 is opposite that expected from the corresponding 15N chemical shifts of the Schiff base nitrogen and may be due to conformational distortion of the chromophore in the C13 = C14-C15 bonds.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 041449737

Download citation: RISBibTeXText

Related references

Structure of a retinal chromophore of dark-adapted middle rhodopsin as studied by solid-state nuclear magnetic resonance spectroscopy. Biophysics and Physicobiology 18: 177-185, 2021

Isomeric composition of retinal chromophore in dark-adapted bacteriorhodopsin. Journal of Biochemistry 82(6): 1599-1604, 1977

Vibrational analysis of the 13-cis-retinal chromophore in dark-adapted bacteriorhodopsin. Journal of Physical Chemistry (1952) 91(4): 804-819, 1987

A Fourier-transform Raman study of the temperature dependence of chromophore conformation in light-adapted and dark-adapted bacteriorhodopsin. Spectrochimica Acta Part A: Molecular Spectroscopy 50(11): 1937-1942, 1994

Refinement of the geometry of the retinal binding pocket in dark-adapted bacteriorhodopsin by heteronuclear solid-state NMR distance measurements. Biochemistry 39(33): 10066-10071, 2000

Ultrafast photochemistry of light-adapted and dark-adapted bacteriorhodopsin: effects of the initial retinal configuration. Journal of Physical Chemistry. B 116(35): 10444-10452, 2012

Vibrational analysis of the all trans retinal chromophore in light adapted bacteriorhodopsin. Journal of the American Chemical Society 109(10): 3108-3125, 1987

Differences in the water distribution between the light adapted and dark adapted state of bacteriorhodopsin. Biological Chemistry Hoppe-Seyler 368(10): 1248, 1987

Photoconversion from the light-adapted to the dark-adapted state of bacteriorhodopsin. Biophysical Journal 48(2): 201-208, 1985

Photoreceptor rhodopsin Determination of the full structure and conformation of 11-cis retinal in the dark adapted state and after photoactivation by novel solid state NMR. Biophysical Journal 76(1 Part 2): A276, 1999

The chromophore retinal in bacteriorhodopsin does not change its attachment site, lysine 216, during proton translocation and light-dark adaptation. European Biophysics Journal 10(4): 211-227, 1984

Deuterium solid-state nuclear magnetic resonance studies of methyl group dynamics in bacteriorhodopsin and retinal model compounds: Evidence for a 6-s-trans chromophore in the protein. Biochemistry 33(11): 3280-3286, 1994

Light adaptation of dark-adapted bacteriorhodopsin studied by nanosecond time-resolved absorption spectroscopy. Photochemistry and Photobiology 56(6): 1013-1018, 1992

Solid-state 13C NMR of the retinal chromophore in photointermediates of bacteriorhodopsin: characterization of two forms of M. Biochemistry 28(1): 237-243, 1989

Photocycles of bacteriorhodopsin in light- and dark-adapted purple membrane studied by time-resolved absorption spectroscopy. Biophysical Journal 56(4): 693-706, 1989