+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Susceptibility of 3H-thymidine-prelabelled Leishmania donovani amastigotes and promastigotes to the cytotoxic activity of peritoneal, splenic and liver macrophages

Susceptibility of 3H-thymidine-prelabelled Leishmania donovani amastigotes and promastigotes to the cytotoxic activity of peritoneal, splenic and liver macrophages

Immunobiology 179(2-3): 202-213

C57BL/6 macrophage populations from spleen and liver, the main organs for the manifestation of visceral leishmaniasis, were investigated for their ability to perform spontaneous phagocytosis-associated killing of 3H-thymidine (3H-TdR)-prelabelled L. donovani amastigotes and promastigotes. The results showed that organ macrophages from spleen and liver killed L. donovani amastigotes and promastigotes spontaneously with high efficiency. This consistent finding was first detectable at 2-3 h, and the reaction was completed at 12 h. This type of killing was strongly enhanced when spleen and liver macrophages were activated. This phagocytosis-associated killing mechanism may contribute, to a large extent, in maintaining the infection under control in vivo, by drastically reducing the amount of parasites that is required to establish intracellular parasitism. To be able to assay phagocytosis-associated destruction of both promastigotes and amastigotes, a reproducible system for the production in vitro of Leishmania donovani amastigotes by the macrophage cell-line J774 was developed. The DNA of the Leishmania amastigotes was labelled with 3H-TdR with high efficiency. The spontaneous label release of prelabelled L. donovani amastigotes was comparable to that of prelabelled promastigotes over an assay period of 24 h.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 041508711

Download citation: RISBibTeXText

PMID: 2793202

DOI: 10.1016/s0171-2985(89)80017-8

Related references

A study of the differential respiratory burst activity elicited by promastigotes and amastigotes of Leishmania donovani in murine resident peritoneal macrophages. Immunology 53(2): 345-355, 1984

Splenic macrophage precursor cells from leishmania donovani infected mice as cytotoxic effectors against promastigotes and amastigotes. Immunobiology 178(1-2): 94, 1988

Anti-parasite activity of nucleoside analogues: the metabolism of carbocyclic inosine in promastigotes of Leishmania tropica and Leishmania donovani and its activity against amastigotes of Leishmania donovani in vitro. Nucleic Acids Symposium Series 1986(17): 149-151, 1986

The cytotoxic effects of diketopiperaizes against Leishmania donovani promastigotes and amastigotes. Medicinal Chemistry Research 22(7): 3452-3458, 2013

Indolylquinoline derivatives are cytotoxic to Leishmania donovani promastigotes and amastigotes in vitro and are effective in treating murine visceral leishmaniasis. Journal of Antimicrobial ChemoTherapy 43(3): 359-366, 1999

RNA editing and mitochondrial activity in promastigotes and amastigotes of Leishmania donovani. International Journal for Parasitology 39(6): 635-644, 2009

Uptake of promastigotes of a lizard Leishmania sp. and Leishmania donovani by mouse peritoneal macrophages. Acta Tropica 40(1): 89-91, 1983

In vitro activity of 10-deacetylbaccatin III against Leishmania donovani promastigotes and intracellular amastigotes. Planta Medica 73(10): 1081-1088, 2007

Leishmania donovani: a chemically defined medium suitable for cultivation and cloning of promastigotes and transformation of amastigotes to promastigotes. Journal of Protozoology 374: 277-279, 1990

Fumarate reductase activity in Trypanosoma cruzi epimastigotes and amastigotes and in Leishmania donovani promastigotes. American Journal of Tropical Medicine & Hygiene 47(4 Suppl. ): 120, 1992

Antimicrobial activity of gentamicin sulfate complex against Leishmania major, L donovani, and L aethiopia amastigotes and promastigotes. Program & Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 33: 257, 1993

Leishmania donovani promastigote glycoconjugates inhibit internalization of amastigotes into balb c peritoneal macrophages. Memorias do Instituto Oswaldo Cruz 85(Suppl. 1): 60, 1990

Effect of bioamines on uptake of promastigotes of Leishmania donovani by hamster peritoneal macrophages. Journal of Medical Microbiology 36(4): 283-287, 1992

Pentamidine uptake in Leishmania donovani and Leishmania amazonensis promastigotes and axenic amastigotes. Biochemical Journal 315: 631-634, 1996

Heparin enhances the interaction of infective Leishmania donovani promastigotes with mouse peritoneal macrophages. Journal of Immunology Baltimore 148(9): 2879-2886, 1992