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Synthesis of diadenosine 5',5'''-P1,P4-tetraphosphate by organellar and cytoplasmic phenylalanyl-tRNA synthetases of Euglena gracilis

Krauspe, R.; Parthier, B.; Wasternack, C.

Febs Letters 235(1-2): 275-277

1988


ISSN/ISBN: 0014-5793
PMID: 3402601
DOI: 10.1016/0014-5793(88)81278-x
Accession: 041521993

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Purified phenylalanyl-tRNA synthetases present in chloroplasts, mitochondria and cytoplasm of green and bleached Euglena gracilis strains, respectively, are able to synthesize diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A). Ap4A synthesis is strictly dependent on zinc ions. This is the first evidence that chloroplasts should be able to synthesize Ap4A. Synthesis of Ap4A by phenylalanyl-tRNA synthetases of the three compartments of a plant cell or by other enzymes such as Ap4A phosphorylase is discussed.

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