+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Systematic generation of sequence-tagged sites for physical mapping of human chromosomes: application to the mapping of human chromosome 7 using yeast artificial chromosomes



Systematic generation of sequence-tagged sites for physical mapping of human chromosomes: application to the mapping of human chromosome 7 using yeast artificial chromosomes



Genomics 11(3): 548-564



Basic to the development of long-range physical maps of DNA are the detection and localization of landmarks within recombinant clones. Sequence-tagged sites (STSs), which are short stretches of DNA that can be specifically detected by the polymerase chain reaction (PCR), can be used as such landmarks. Our interest is to construct physical maps of whole human chromosomes by localizing STSs within yeast artificial chromosome (YAC) clones. Here we describe a generalized strategy for the systematic generation of large numbers of STSs specific for human chromosome 7. These STSs can be detected by PCR assays developed following the sequencing of anonymous pieces of chromosome 7 DNA, which was derived from flow-sorted chromosomes or from lambda clones made from DNA of a human-hamster hybrid cell line. Our approach for STS generation is tailored for the development of PCR assays capable of screening a large YAC library. In this study, we report the generation of 100 new STSs specific to human chromosome 7.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 041525378

Download citation: RISBibTeXText

PMID: 1837788

DOI: 10.1016/0888-7543(91)90062-j


Related references

Physical mapping of human chromosomes: generation of chromosome-specific sequence-tagged sites. Methods in molecular genetics(1): 192-210, 1993

Mapping human chromosomes by walking with sequence-tagged sites from end fragments of yeast artificial chromosome inserts. Genomics. 14(2): 241-248, 1992

Somatic cell hybrids, sequence-tagged sites, simple repeat polymorphisms, and yeast artificial chromosomes for physical and genetic mapping of proximal 17p. Genomics 13(3): 551-559, 1992

Physical mapping of human chromosome 22 using yeast artificial chromosomes. American Journal of Human Genetics 49(4 Suppl.): 383, 1991

Physical mapping of yeast artificial chromosomes yacs on human chromosomes 21 and 18 by fluorescence in situ hybridization. American Journal of Human Genetics 49(4 Suppl.): 382, 1991

Generation of chromosome 21 specific stss for physical mapping using yeast artificial chromosomes. American Journal of Human Genetics 49(4 Suppl.): 388, 1991

Characterization and chromosomal assignment of yeast artificial chromosomes containing human 3p13-p21-specific sequence tagged sites. Cancer Genetics & Cytogenetics. 81(1): 1-12, 1995

Sequence tagged sites of microclones obtained by microdissection of a human chromosomal region 11q23 and isolation of yeast artificial chromosomes. Japanese Journal of Human Genetics 39(2): 249-254, 1994

Generation of 28 sequence-tagged sites (STSs) from yeast artificial chromosome (YAC) clones anchored at human chromosome 21q22.1 region. Dna Sequence 5(4): 239-243, 1995

In vitro fragmentation of yeast chromosomes and yeast artificial chromosomes at artificially inserted sites and applications to genome mapping. Adolph, Kenneth W Methods in Molecular Genetics; Microbial gene techniques 81-107, 1995

Physical mapping of sequence tagged sites on human chromosome 11 using the PRINS-CARD method. American Journal of Human Genetics 61(4 Suppl. ): A246, 1997

Physical mapping of rice chromosome 1 with yeast artificial chromosomes (YACs). Dna Research 3(5): 291-296, 1996

Physical mapping of the human T-cell receptor beta gene complex, using yeast artificial chromosomes. Immunogenetics 41(6): 337-342, 1995

Physical mapping of yeast artificial chromosomes containing sequences from the human beta-globin gene region. Genomics 10(4): 976-984, 1991

Human chromosome 16 physical map: mapping of somatic cell hybrids using multiplex PCR deletion analysis of sequence tagged sites. Genomics 10(4): 1047-1052, 1991