+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Techniques of DNA hybridization detect small numbers of mycobacteria with no cross-hybridization with non-mycobacterial respiratory organisms



Techniques of DNA hybridization detect small numbers of mycobacteria with no cross-hybridization with non-mycobacterial respiratory organisms



American Review of Respiratory Disease 131(5): 760-763



The traditional methods used in identifying mycobacteria, such as acid-fast bacillus stains and culture, are often time-consuming, insensitive, and nonspecific. As part of an ongoing program to improve diagnosis and characterization of mycobacteria, we have found that deoxyribonucleic acid (DNA) hybridization techniques using isotopically labeled, single-stranded, total DNA can be used to detect as little as 10(-4) micrograms of Mycobacterium tuberculosis (MTb) DNA. This amount of DNA represents approximately 2 X 10(4) genomes. We have also shown the MTb DNA is sufficiently different from the DNA of non-mycobacterial microorganisms such that cross-hybridization with MTb DNA does not occur under the hybridization conditions we employed. We speculate that DNA hybridization techniques may allow the rapid, sensitive, and specific identification of mycobacteria.

(PDF emailed within 0-6 h: $19.90)

Accession: 041539051

Download citation: RISBibTeXText

PMID: 3923880

DOI: 10.1164/arrd.1985.131.5.760


Related references

Investigation of mycobacteria in crohn's disease tissue by southern blotting and dna hybridization with cloned mycobacterial genomic dna probes from a crohn's disease isolated mycobacteria. Gut 29(9): 1222-1228, 1988

Evaluation of INNO-LiPA MYCOBACTERIA v2: improved reverse hybridization multiple DNA probe assay for mycobacterial identification. Journal of Clinical Microbiology 41(9): 4418-4420, 2003

Hybridization techniques and mycobacteria. Evaluation in hospital practice. Enfermedades Infecciosas Y Microbiologia Clinica 10(2): 71-74, 1992

Biotinylated probes to detect Leptospira interrogans on dot blot hybridization or by in situ hybridization. Letters in Applied Microbiology 12(5): 171-176, 1991

Using in situ hybridization and PFGE southern hybridization to detect translocation breakpoints in a BOR/TRPS patient cell line. American Journal of Human Genetics 55(3 SUPPL ): A370, 1994

Species of environmental mycobacteria differ in their abilities to grow in human, mouse, and carp macrophages and with regard to the presence of mycobacterial virulence genes, as observed by DNA microarray hybridization. Applied and Environmental Microbiology 74(1): 275-285, 2007

Immunologic techniques to detect small amounts of specific mycobacterial antigens. Federation Proceedings 37(6): 1376, 1978

Use of dna hybridization techniques to detect agrobacterium tumefaciens t dna in symptomless vitis rotundifolia vines. Phytopathology 78(5): 629-630, 1988

Polymerase chain reaction and reverse cross blot hybridization assay for detection of mycobacterial DNA in lupus vulgaris. Dermatology 195(3): 293-296, 1997

Screening bacterial colonies by hybridization: small numbers. Csh Protocols 2006(1): -, 2006

Nucleic acid labeling and hybridization techniques for southern blots and in situ hybridization. Journal of Cell Biology 115(3 PART 2): 89A, 1991

Fluorescence in situ hybridization, comparative genomic hybridization, and other molecular biology techniques in the analysis of effusions. Diagnostic Cytopathology 33(5): 294-299, 2005

Basic Molecular Techniques Genomic DNA isolation, southern blotting and hybridization Hybridization with radioactive probes. Clark, M. S. Plant Molecular Biology: A Laboratory Manual: 26-40, 1997

Localization of repetitive dna in cereals by in situ hybridization cross hybridization among wheat rye barley and oat. Plant Systematics and Evolution 129(4): 261-268, 1978