+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The 5' end of coronavirus minus-strand RNAs contains a short poly(U) tract

The 5' end of coronavirus minus-strand RNAs contains a short poly(U) tract

Journal of Virology 65(11): 6331-6333

A radiolabeled oligodeoxynucleotide primer that anneals near the common 5' end of bovine coronavirus minus-strand RNAs was extended with reverse transcriptase, and a major product suggesting poly(U) tracts of 8 to 20 nucleotides was found. The extended primer molecules were ligated head to tail, amplified by the polymerase chain reaction, cloned, and sequenced, and poly(U) tracts of 9 to 26 nucleotides were found. Poly(A) tails of 100 to 130 nucleotides on the 3' end of coronavirus plus-strand mRNAs and genome must, therefore, be generated by a mechanism that uses only a short poly(U) template. This pattern contrasts with that of other cytoplasmic, polyadenylated, plus-strand animal RNA viruses which utilize a full-length poly(U) template for poly(A) synthesis.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 041552052

Download citation: RISBibTeXText

PMID: 1920635

Related references

Coronavirus subgenomic minus-strand RNAs and the potential for mRNA replicons. Proceedings of the National Academy of Sciences of the United States of America 86(14): 5626-5630, 1989

Coronavirus genomic and subgenomic minus-strand RNAs copartition in membrane-protected replication complexes. Journal of Virology 71(10): 7744-7749, 1997

Identification of the cis-acting signal for minus-strand RNA synthesis of a murine coronavirus: implications for the role of minus-strand RNA in RNA replication and transcription. Journal of Virology 68(12): 8131-8140, 1994

Altered balance of the synthesis of plus- and minus-strand RNAs induced by RNAs 1 and 2 of alfalfa mosaic virus in the absence of RNA 3. Virology 124(1): 75-85, 1983

Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis. Journal of Virology 57(1): 328-334, 1986

Role of subgenomic minus-strand RNA in coronavirus replication. Archives of Virology. Supplementum 9: 173-180, 1994

Minus-strand copies of replicating coronavirus mRNAs contain antileaders. Journal of Virology 65(1): 320-325, 1991

Natural minus-strand RNAs of alfalfa mosaic virus as in vitro templates for viral RNA polymerase. 3'-terminal non-coded guanosine and coat protein are insufficient factors for full-size plus-strand synthesis. Archives of Virology 146(3): 571-588, 2001

Northern analysis of viral plus- and minus-strand RNAs. Current Protocols in Microbiology Chapter 16: Unit 16e.3, 2007

Different mechanisms of recognition of bacteriophage Qb plus and minus strand RNAs by Qb replicase. Journal of Molecular Biology 232: 2-21, 1993

Interactions of plus and minus strand leader RNAs of the New Jersey serotype of vesicular stomatitis virus with the cellular La protein. Virology 135(1): 65-73, 1984

Replicase-binding sites on plus- and minus-strand brome mosaic virus RNAs and their roles in RNA replication in plant cells. Journal of Virology 78(24): 13420-9, 2004

Effect of defective interfering particles on plus- and minus- strand leader RNAs in vesicular stomatitis virus-infected cells. Journal of Virology 35(3): 704-709, 1980

Poly uridylate in semliki forest virus minus strand rna. Abstracts of the Annual Meeting of the American Society for Microbiology 76: S274, 1976

Different mechanisms of recognition of bacteriophage Q beta plus and minus strand RNAs by Q beta replicase. Journal of Molecular Biology 232(2): 512-521, 1993