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The effect of some anti-inflammatory agents on elastase release from neutrophils in-vitro

Adeyemi, E.O.; Chadwick, V.S.; Hodgson, H.J.

Journal of Pharmacy and Pharmacology 42(7): 487-490

1990

ISSN/ISBN: 0022-3573
PMID: 1980289
DOI: 10.1111/j.2042-7158.1990.tb06601.x
Accession: 041631891
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In view of the potential role of released polymorphonuclear leucocyte elastase in causing tissue damage, the effect of commonly used anti-inflammatory drugs on elastase release from neutrophils has been studied in-vitro. Elastase release from neutrophils exposed to the synthetic bacterial cell wall peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (10(-6) M) was quantitated using a radiometric immunoassay and a functional assay of elastase. Prednisolone and non-steroidal anti-inflammatory drugs inhibited elastase release at concentrations from 0.1 mM-0.1 nM. No inhibition by sulphosalicylic acid, D-penicillamine or chloroquine sulphate was observed. The clinical relevance of these findings is discussed.

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