+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The human cytokine I-309 is a monocyte chemoattractant



The human cytokine I-309 is a monocyte chemoattractant



Proceedings of the National Academy of Sciences of the United States of America 89(7): 2950-2954



The human cytokine I-309 is a small glycoprotein secreted by activated T lymphocytes and structurally related to a number of inflammatory cytokines. To investigate the biological activities of I-309 protein, we produced a stable Chinese hamster ovary cell transfectant, CDI.10, which constitutively secretes I-309 protein into culture supernatant. Affinity chromatography on a heparin-Sepharose matrix followed by reverse-phase HPLC was used to purify to homogeneity a glycoprotein doublet of 15-16 kDa from culture supernatant. Biochemical analysis showed the purified recombinant I-309 glycoprotein to be indistinguishable from the natural I-309 glycoprotein constitutively secreted by the T-cell line IDP2. Purified recombinant I-309 stimulated migration of human monocytes but not neutrophils when tested by in vitro chemotaxis assay. Furthermore, the purified protein transiently increased cytoplasmic free calcium concentration in human peripheral blood monocytes but did not do so in lymphocytes or neutrophils. These results demonstrate that the I-309 gene encodes an inflammatory mediator that specifically stimulates human monocytes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 041664474

Download citation: RISBibTeXText

PMID: 1557400

DOI: 10.2307/2359011


Related references

Mechanical stretch induces monocyte chemoattractant activity via an NF-kappaB-dependent monocyte chemoattractant protein-1-mediated pathway in human mesangial cells: inhibition by rosiglitazone. Journal of the American Society of Nephrology 16(3): 688-696, 2005

Production of monocyte chemoattractant protein-1 and cytokine-induced neutrophil chemoattractant-1 in rat brain is stimulated by intracerebroventricular administration of an endothelin ETB receptor agonist. Neuroreport 18(12): 1275-1279, 2007

Upregulation of the proinflammatory cytokine-induced neutrophil chemoattractant-1 and monocyte chemoattractant protein-1 in rats' intestinal anastomotic wound healing--does it matter?. Asian Journal of Surgery 37(2): 86-92, 2014

Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE. American Journal of Pathology 136(6): 1229-1233, 1990

Monocyte chemoattractant protein-3, but not monocyte chemoattractant protein-2, is a functional ligand of the human monocyte chemoattractant protein-1 receptor. Journal of Immunology 154(12): 6511-6517, 1995

Human PBMC transmigrate across a coculture of human endothelial cells and astrocytes in response to cytokine-induced monocyte chemoattractant protein. FASEB Journal 12(4): A183, 1998

Abnormalities in monocyte recruitment and cytokine expression in monocyte chemoattractant protein 1-deficient mice. Journal Of Experimental Medicine. 187(4): 601-608,. 16, 1998

Cytokine-induced production of monocyte chemoattractant protein-1 by cultured human mesangial cells. Journal of Immunology 148(7): 2148-2153, 1992

Monocyte chemoattractant protein-1 regulates adhesion molecule expression and cytokine production in human monocytes. Journal of Immunology 148(8): 2423-2428, 1992

A synthetic peptide of human C-reactive protein enhances expression of monocyte chemoattractant protein 1 mRNA and monocyte chemoattractant bioactivity in murine pulmonary leukocytes. Journal of Immunology 150(8 Part 2): 133A, 1993

Inhibition of monocyte chemoattractant protein-1 expression in cytokine-treated human lung epithelial cells by thiazolidinedione. Chest 120(4): 1293-1300, 2001

Thiazolidinedione inhibits the production of monocyte chemoattractant protein-1 in cytokine-treated human vascular endothelial cells. FEBS Letters 454(1-2): 27-30, 1999

Detection of monocyte chemoattractant protein-1 in human atherosclerotic lesions by an anti-monocyte chemoattractant protein-1 monoclonal antibody. Human Pathology. 24(5): 534-539, 1993