The immunobiology of M-proteins of Streptococcus pyogenes. I. Isolation and purification of M-proteins from group a streptococci (Streptococcus pyogenes) type 1
Kühnemund, O.; Köhler, W.
Allergie und Immunologie 22(2): 171-178
1976
ISSN/ISBN: 0323-4398 PMID: 136885 Accession: 041666844
Streptococci of group A, type 1, are lysed by use of phage associated lysin, which is characterized as a N-Acetyl-D-glucosaminidase. This enzyme has no proteolytic activity. Type specific protein antigens are isolated and purified by ion exchange chromatography on CM-cellulose and DEAE-cellulose of the crude extract, concentrated by precipitation with ammonium sulfate. The immunization tests are carried out on monkeys and rabbits.
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