+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The in vitro suppression of antigen- or mitogen-induced DNA synthesis by murine spleen cells after the addition of freshly prepared syngeneic cells



The in vitro suppression of antigen- or mitogen-induced DNA synthesis by murine spleen cells after the addition of freshly prepared syngeneic cells



Journal of Immunology 116(2): 379-386



The suppression of antigen- and mitogen-induced DNA synthesis by murine spleen cells in vitro was investigated. It appears that cultures receiving two signals exhibit marked suppression of DNA synthesis. The addition of KLH, PPD, Con A, PHA, or LPS to 72-hr-lod cultures of KLH-stimulated murine spleen cells resulted in the suppression of DNA synthesis assayed at 144 hr. When small numbers of freshly prepared KLH-PPD SC or NSC were added to these cultures at 72 hr the suppression of DNA synthesis was abrogated. However, the addition of larger numbers of KLH-PPD SC or NSC resulted in increased suppression of DNA synthesis. Large numbers of KLH-PPD SC or NSC could substitute for the second stimulant (KLH, PPD, Con A, PHA, or LPS) in suppressing DNA synthesis. The addition of fresh cells obtained from KLH-PPD-immunized mice were more effective in eliciting the subsequent suppression than were cells obtained from non-immunized mice. Cells obtained 30 to 75 days post immunization were most effective in suppressing DNA synthesis.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 041670862

Download citation: RISBibTeXText

PMID: 765398


Related references

The suppression of dna synthesis in cultures of keyhole limpet hemo cyanin stimulated murine spleen cells which receive freshly prepared syngeneic cells at 72 hours. Federation Proceedings 36(3): 1270, 1977

The suppression of antigen induced dna synthesis in vitro by murine spleen cells after addition of fresh syngenic spleen cells. Federation Proceedings 33(3 Part 1): 649, 1974

The suppression of antigen induced dna synthesis in vitro by murine spleen cells following the addition of varying concentrations of fresh syngenic spleen cells and mitogens. Federation Proceedings: 1021, 1975

Suppression of mitogen induced proliferation of normal spleen cells by spleen cells from syngeneic chickens inoculated with human adenovirus type 6. Acta Microbiologica Academiae Scientiarum Hungaricae 27(3): 230, 1980

Suppression of dna synthesis in murine spleen cells with culture supernatant fluids from nonstimulated keyhole limpet hemo cyanin sensitive murine spleen cells. Federation Proceedings 36(3): 1271, 1977

Suppression of In Vitro Antigen-Induced Release of Interleukin-1 by Spleen Cells. Rheumatology Xxiv (Suppl 1): 91-93, 1985

Suppression of in vitro antigen induced release of interleukin 1 by spleen cells. British Journal Of Rheumatologyppl. 1: 91-93, 1985

Genetic control of contract hypersensitivity. II. Suppressor cells induced by intravenous injection of DNP-coupled syngeneic spleen cells possessing thy-1 antigen, I-J determinant, Lyt-2+, 3+ antigen, FcR- and DNP-membrane binding receptor on their surfaces. Microbiology and Immunology 25(1): 63-70, 1981

Immunotherapy of a murine colon cancer with syngeneic spleen cells, immune RNA and tumor antigen. International Journal of Cancer 29(1): 107-112, 1982

Mycobacterial di-O-acyl-trehalose inhibits mitogen- and antigen-induced proliferation of murine T cells in vitro. Clinical and Diagnostic Laboratory Immunology 8(6): 1081-1088, 2001

Legionella pneumophila induced suppression of early blastogenic responsiveness of murine spleen cells to specific and nonspecific stimulation in vitro. Current Microbiology 16(3): 123-127, 1987

Dynamics of antigen and mitogen-induced responses in murine schistosomiasis japonica: in vitro comparison between hepatic granulomas and splenic cells. Journal of Immunology 127(1): 115-120, 1981

In vitro induction of cytotoxicity against syngeneic mastocytoma and its suppression by spleen and thymus cells from tumor-bearing mice. Journal of Immunology 116(2): 288-293, 1976

T-cell responses induced by the parenteral injection of antigen-modified syngeneic cells. III. Dissociation of primed cytolytic T-cell and efferent suppressor-T-cell activity following intravenous injection of trinitrophenol-modified syngeneic spleen cells. Cellular Immunology 86(2): 308-316, 1984

In vitro proliferative response of BALB/c mouse spleen cells stimulated with trinitrophenylated syngeneic spleen cells. Immunology 29(5): 875-884, 1975