+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The polymerase gene of corona- and toroviruses: evidence for an evolutionary relationship

The polymerase gene of corona- and toroviruses: evidence for an evolutionary relationship

Advances in Experimental Medicine and Biology 276: 307-316

In this paper we demonstrate that the organization of the polymerase gene of toroviruses and coronaviruses is similar. The polymerase gene of both virus families consists of at least two large ORFs (1a and 1b). Four domains of conserved amino acid sequences have been identified in nearly identical positions in the 3' ORF of the pol gene of toroviruses and coronaviruses. The most 3' conserved domain which is still unique for these viruses encodes a 33-kDA protein in MHV-A59, which is cleaved from a precursor protein. Expression of ORF1b of the pol gene of both virus families occurs by ribosomal frameshifting. A predicted stem-loop structure and pseudoknot are conserved in the ORF1a/ORF1b overlap of toro- and coronaviruses. On the basis of these results we postulate that toro- and coronaviruses are ancestrally more related to each other than to other families of positive stranded RNA viruses.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 041716290

Download citation: RISBibTeXText

PMID: 1966417

Related references

Molecular evolution of corona- and toroviruses. Advances in Experimental Medicine and Biology 473: 61-72, 1999

Structure, function and evolution of the hemagglutinin-esterase proteins of corona- and toroviruses. Glycoconjugate Journal 23(1-2): 59-72, 2006

Detection and molecular characterisation of bovine corona and toroviruses from Croatian cattle. Bmc Veterinary Research 11: 202, 2015

Design and validation of consensus-degenerate hybrid oligonucleotide primers for broad and sensitive detection of corona- and toroviruses. Journal of Virological Methods 177(2): 174-183, 2011

Evidence for genetic relationship between RNA and DNA viruses from the sequence homology of a putative polymerase gene of bluetongue virus with that of vaccinia virus: conservation of RNA polymerase genes from diverse species. Nucleic Acids Research 16(24): 11759-11767, 1988

Whole genome analysis of Japanese bovine toroviruses reveals natural recombination between porcine and bovine toroviruses. Infection Genetics and Evolution 38: 90-95, 2016

Evolutionary relationship between Old World West Nile virus strains. Evidence for viral gene flow between Africa, the Middle East, and Europe. Virology 315(2): 381-388, 2003

The carboxyl terminal part of the putative berne virus polymerase is expressed by ribosomal frameshifting and contains sequence motifs which indicate that toroviruses and coronaviruses are evolutionarily related. Nucleic Acids Research 18(15): 4535-4542, 1990

Evolutionary relatedness of the predicted gene product of RNA segment 2 of the tick-borne Dhori virus and the PB1 polymerase gene of influenza viruses. Virology 182(1): 1-7, 1991

Evolutionary stasis of M1 gene of human influenza A viruses and the possibility of their subtyping by restriction analysis of M1 gene polymerase chain reaction product. Acta Virologica 41(4): 231-239, 1997

Analysis of the transcript of the herpes simplex virus DNA polymerase gene provides evidence that polymerase expression is inefficient at the level of translation. Journal of Virology 62(6): 2007-2015, 1988

Genetic variation and co-evolutionary relationship of RNA polymerase complex segments in influenza A viruses. Virology 511: 193-206, 2017

Sequence analysis of the clpG gene, which codes for surface antigen CS31A subunit: evidence of an evolutionary relationship between CS31A, K88, and F41 subunit genes. Journal of Bacteriology 173(23): 7673-7683, 1991

Evolutionary history of the poly(ADP-ribose) polymerase gene family in eukaryotes. Bmc Evolutionary Biology 10: 308, 2010

Evolutionary conservation of an excision repair gene The use of degenerate polymerase chain reaction. Mutagenesis 7(5): 392-393, 1992