+ Translate

The prevalence of ouabain-resistant variants after mutagen treatment. Lack of correlation between the frequency of variant expression and the metastatic phenotype

, : The prevalence of ouabain-resistant variants after mutagen treatment. Lack of correlation between the frequency of variant expression and the metastatic phenotype. Invasion & Metastasis 8(2): 73-86

In order to test the hypothesis that tumor cells with greater malignant potential should have an increased sensitivity to mutagens, four individual murine cell lines, each paired for their metastatic and nonmetastatic potentials, were compared with respect to the prevalence at which ouabain-resistant mutants could be obtained after treatment of the cells with the mutagen MNNG. No increase in the prevalence of induced mutation in metastatic cells was observed; and, in fact, in two cases (h-ras-transfected 3T3 and SP1 cells), nonmetastatic cells had a higher prevalence of mutations after MNNG treatment than did their metastatic counterparts. We suggest that the absolute level of genomic instability, measured by this means, is not critical to malignant potential and tumor progression.

(PDF 0-2 workdays service)

Accession: 041720987

PMID: 3360593

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Zackon I.; Sorenson G.D.; Davis B.H., 1992: Lack of correlation of integrin expression with the metastatic phenotype in transfected mouse fibroblasts. Proceedings of the American Association for Cancer Research Annual Meeting 33: 63

Mracek M.; Blumauerova M.; Paleckova F.; Hostalek Z., 1969: Regulation of biosynthesis of secondary metabolites induction of variants in streptomyces aureofaciens and the specificity of mutagens gamma radiation mutagen uv irradiation mutagen x ray mutagen n methyl n nitroso n nitro guanidine mutagen nitrosomethyl urea mutagen ethyl methanesulfonate mutagen nitrous acid mutagen hydroxylamine mutagen formaldehyde mutagen nitrogen mustard mutagen. Mutation Research 7(1): 19-24

Lin, J.; Swan, G.E.; Shields, P.G.; Benowitz, N.L.; Gu, J.; Amos, C.I.; de Andrade, M.; Spitz, M.R.; Wu, X., 2007: Mutagen sensitivity and genetic variants in nucleotide excision repair pathway: genotype-phenotype correlation. The rationale behind gene-disease association studies is that genetic variants (polymorphisms) result in alterations in intermediate phenotypes. However, genotype-phenotype correlations have not been established for most polymorphisms. In this st...

Seiter S.; Hofmann M.; Herrlich P.; Zoeller M., 1991: Expression of variant cd44 transfers the metastatic phenotype and antibodies against variant cd44 inhibit metastatic spread in lymphatic tissue. Immunobiology 183(3-4): 221-222

Kalebic T., 1986: Induction of the metastatic phenotype by transfection with ha ras oncogene susceptibility of different cell types to fully malignant transformation and the lack of correlation between level of gene expression and degree of malignancy. Uicc (Union Internationale Contre Le Cancer, International Union Against Cancer) 14th International Cancer Congress, Budapest, Hungary, Aug 21-27, Abstracts, Lectures, Symposia And Free Communications, Vols 1, 2, 3, Late Abstracts, And Register Xvi+479p (Vol 1); Xvi+298p (Vol 2); Xvi+531p (Vol 3); 15p (Late Abstracts); 40p (Register) S Karger Ag: Basel, Switzerland; New York, N Y , Usa; Akademiai Kiado: Budapest, Hungary Paper 15

Ezrailson, E.G.; Potter, J.D.; Michael, L.; Schwartz, A., 1977: Positive inotropy induced by ouabain, by increased frequency, by X537A (RO2-2985), by calcium and by isoproterenol: the lack of correlation with phosphorylation of TnI. Journal of Molecular and Cellular Cardiology 9(8): 693-698

Marchand, M.; Caspar, P.; Boon, T., 1983: Increased frequency of immunogenic variants obtained by repeated mutagen treatment of mouse mastocytoma P815. A previous report from this laboratory demonstrated that treatment of mouse mastocytoma P815 with the mutagen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) produces tumor cell variants that are unable to form tumors in syngeneic animals. We exa...

Carlucci, M.J.; Scolaro, L.A.; Damonte, E.B., 2002: Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: lack of correlation between drug susceptibility and syn phenotype. Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii were recently identified as potent and selective inhibitors of herpes simplex virus types 1 and 2 (HSV-1 and -2). The mu/nu-carrageenan 1C3 was t...

Kashleva E.A.; Deichman G.I., 1988: Metastatic phenotype of syrian hamster cells transformed by rous sarcoma viruses and the frequency of the occurrence of cells with such phenotype in the populations of low metastatic and high metastatic cell lines. Tsitologiya 30(9): 1132

Ezrailson, E.G.; Potter, J.D.; Michael, L.; Schwartz, A., 1977: Positive inotropy induced by ouabain by increased frequency by x 537a ro 2 2985 lasalocid by calcium and by isoproterenol the lack of correlation with phosphorylation of inhibitory subunit of tropin. Isoproterenol (5 .times. 10-6 M) was perfused into a cat heart Langendorff preparation until a maximum positive inotropic response was obtained. Ouabain (9.6 .times. 10-7 M), increased frequency of stimulation, an ionophore RO2-2985 (X537A) [lasal...