+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The primary structure of alpha-amylase inhibitor Z-2685 from Streptomyces parvullus FH-1641. Sequence homology between inhibitor and alpha-amylase

, : The primary structure of alpha-amylase inhibitor Z-2685 from Streptomyces parvullus FH-1641. Sequence homology between inhibitor and alpha-amylase. Biological Chemistry Hoppe-Seyler 366(12): 1161-1168

The native and oxidized alpha-amylase inhibitor Z-2685, isolated from the culture medium of Streptomyces parvullus FH-1641, and its overlapping cleavage products were degraded by the automatic Edman technique. Digestion was carried out with pepsin, thermolysin and trypsin. The alpha-amylase inhibitor is a polypeptide consisting of 76 amino acids with a molecular mass of 8 129 Da. With the exception of methionine and lysine, all naturally occurring amino acids are present. It is interesting that identical regions exist, in particular the sequence Trp-Arg-Tyr common to all four known microbial inhibitor sequences. We believe that the side chains of these three amino acids are important for interacting with the alpha-amylase molecule. Computer alignment enabled us to show a possible binding region in the alpha-amylase molecule which might react with the inhibitors. Furthermore, homology exists to mammalian alpha-amylases. This result is explained by the assumption that the inhibitor evolved from a duplication of the original gene of the enzyme.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 041721870

PMID: 3879177

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Spiridonov, A.A.; Satmari, V.V.; Zaporozhan, A.A.; Grozovskiĭ, I.A.; Il'ina, M.B.; Novikova, S.P.; Shekhter, A.B.; Dobrova, N.B., 1989: Studies and clinical experiences in the use of domestic blood vessel prostheses with increased thromboresistance. The experimental and clinical data on the use of new native grafts with increased thromboresistance--the Vitlan and heparinized ones are presented. In all the acute and chronic experiments, the increased thromboresistance of both types of the graf...

Tripoulas N.A.; Samols D., 1986: Development and hormonal regulation of sericin rna in the silkworm bombyx mori. Sericin and fibroin are the major protein products of the terminally differentiated silk gland in the commercial silkworm, Bombyx mori, accounting for more than 90% of the protein in the cocoon. cDNA clones to sericin RNA were isolated and used as...

Meike A., 1988: Deformation of micas in-situ at the high voltage electron microscope. Eos, Transactions, American Geophysical Union 69(29): 731

Yoshida, S.; Ohshima, H.; Kobayashi, S., 1989: Vascularization of the enamel organ in developing molar teeth of rats--scanning electron microscope study of corrosion casts. Vascularization of the enamel organ was demonstrated in the developing upper first molar teeth of rats from the 19-day embryo to 5 days after birth employing the vascular casting/scanning electron microscope method. Capillaries were first observed...

Raneses R.; Grines C.; Almany S.; Timmis G.; O'neill W.W., 1992: Hemodynamic parameters 12 hours after onset of cardiogenic shock can predict survival. Journal of the American College of Cardiology 19(3 SUPPL A): 362A

Farrow, B.; Evers, B.Mark., 2003: Activation of PPARgamma increases PTEN expression in pancreatic cancer cells. The PI3K pathway contributes to the invasive properties and apoptosis resistance that epitomize pancreatic cancers. PPARgamma is a ligand-activated transcription factor with anti-inflammatory and anti-tumor effects; the mechanisms of tumor suppres...

Esener, Z.; Sarihasan, B.; Güven, H.; Ustün, E., 1992: Thiopentone and etomidate concentrations in maternal and umbilical plasma, and in colostrum. We have measured concentrations of etomidate and thiopentone in maternal plasma, umbilical venous plasma and colostrum after induction of anaesthesia in 40 patients undergoing Caesarean section. Mean plasma etomidate concentration declined rapidly...

Mclaren, Rodney A.; Puckett, Jamie L.; Chauhan, Suneet P., 1995: Estimate of birth weight among diabetic patients Comparison of seven sonographic models. American Journal of Obstetrics & Gynecology 172(1 PART 2): 332

Smith, I. M.; Mohammadi, H.; Licence, S. T.; Hill, R., 1977: Experimental fowl typhoid in chicks impaired immunologically by treatment with cyclo phosphamide. Male chicks were injected i.m. with 6 mg of cyclophosphamide daily for 3 consecutive days, the initial dose being given on the day of hatching. This treatment impaired bursal development and lymphopoiesis and no circulating antibodies appeared aft...

Wolfenbarger, D. A., 1998: Critique of need for automatic early spring insecticide applications for suppression program against boll weevil in Lower Rio Grande Valley. Two automatic applications of insecticides in the spring against boll weevils [Anthonomus grandis] in cotton when pinhead squares first develop in each field in the Lower Rio Grande Valley (LRGV) of Texas, USA and Tamaulipas, Mexico, are questione...