+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The primary structure of apolipoprotein A-I from rabbit high-density lipoprotein

, : The primary structure of apolipoprotein A-I from rabbit high-density lipoprotein. European Journal of Biochemistry 160(2): 427-431

The amino acid sequence of rabbit apolipoprotein A-I (apo A-I) has been determined by degradation and alignment of two overlapping sets of peptides obtained from tryptic and staphylococcal digestions. All of the peptides of rabbit apo A-I resulting from digestion by staphylococcal protease were isolated and sequenced except residues 33-37. A digestion with trypsin was employed to find overlapping and missing peptides. The N-terminus of rabbit apo A-I was confirmed by sequencing the intact protein up to 20 residues while the C-terminus was identified through its homology with human apo A-I. The protein contains 241 residues in its single chain. Its primary structure is highly homologous to the reported canine apo A-I (80%) and human apo A-I (78%), but exhibits less similarity with rat apo A-I (60%). Like human apo A-I, rabbit apo A-I contains very little histidine (2) and methionine (1); it does however have two residues of isoleucine. Based on a comparison of the hydrophobic-hydrophilic character of apo A-I residues with that of the two synthetic peptides that activated lecithin: cholesterol acyltransferase (Pownall et al. and Yokoyama et al.), we found that the five segments with the highest corresponding homologies on the protein are located within the N-terminal half. This suggests that the N-terminal half of apo A-I contains the major portion of regions activating lecithin: cholesterol acyltransferase.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 041721873

PMID: 3095115

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Otto, Rafael; De-Faria, Maria-Regina-Meireles; Queiroz, Fabio-Eduardo-De-Campos; De-Moura, Thiago-Augusto; Vitti, Godofredo-Cesar; Cantarella, Heitor, 2007: Yield of sugar cane as affected by fluorine. Heating minerals, roctks, clays and soils in aluminum and iron smelters, and in ceramic, glass, and fertilizer industries release gaseous forms of fluorine into the atmosphere. In an area grown with two varieties of sugar cane (Saccharum officinar...

Lamparter, T., 2006: A computational approach to discovering the functions of bacterial phytochromes by analysis of homolog distributions. Background: Phytochromes are photoreceptors, discovered in plants, that control a wide variety of developmental processes. They have also been found in bacteria and fungi, but for many species their biological role remains obscure. This work conce...

Rieder, H.L.; Zimmermann, H.; Zwahlen, M.; Billo, N.E., 1990: Epidemiology of tuberculosis in Switzerland. Mortality for tuberculosis declined from an average of 7.5% per year in 1952 to 1.7 in 1988, tuberculosis incidence declined by 5 to 6% per year since 1945 to 17.4% in 1988, and the annual risk of infection declined by 11.4% per year since 1945 to...

Helmchen, U.; Kneissler, U.; Churchill, P.; Peters-Haefeli, L.; Schaechtelin, G.; Peters, G., 1972: Plasma renin activity in renal hypertensive rats. Pfluegers Archiv European Journal of Physiology 332(3): 232-238

Ressl, J.; Chrástek, J.; Jandová, R., 1977: Haemodynamic effects of physical training in essential hypertension. The haemodynamic effects of four weeks of daily intensive training on bicycle ergometer were studied in 10 men with essential hypertension of grade II (WHO). Three weeks before training all medication was replaced by placebo. Five days before onse...

Shakeel-Ur-Rehman; Banks, Jm; Brechany, Ey; Muir, Dd; Mcsweeney, Plh; Fox, Pf, 2000: Influence of ripening temperature on the volatiles profile and flavour of Cheddar cheese made from raw or pasteurised milk. Cheddar cheeses were made from raw (R1, R8) or pasteurised (P1, P8) milk and ripened at 1 degree C (P1, R1) or 8 degrees C (P8, R8). Volatile compounds were extracted from 6 month-old cheeses and analysed, identified and quantified by gas chromato...

Escalera, C. R.; Maezawa, A.; Uchida, S.; Kuroda, M., 1993: Effectiveness factors in anaerobic biofilms: a pseudoanalytical equation for parallel and consecutive reactions. The effectiveness factors for an anaerobic biofilm in which a parallel-consecutive reaction occurs were studied theoretically. A pseudo-analytical equation to represent the effectivness factors (Efj) was developed as a function of a dimensionless...

Huon, Ernest, 1931: Research on Variola Vaccine. The experiments recorded in this paper were carried out in collaboration with WURTZ and comprised three series: -(a) attempts to infect calves and asses with freshly collected smallpox virus; (b) the variolisation of bovines by GAUDUCHEAU'S m...

Ash S.A.; Griffin G.E.; Stock M.F., 1989: Non protein energy source in parenterally fed weanling rats influences growth body composition and skeletal muscle protein turnover. Comparison was made between three total parenteral nutrition (TPN) regimens given to young, weanling rats for 5 days. The three isoenergetic and isonitrogenous regimens contained different ratios of glucose to lipid as non-protein energy sources,...

Hoshi M.; Ohta K.; Gotoh Y.; Mori A.; Murofushi H.; Nishida E.; Sakai H., 1990: Map kinase catalyzed phosphorylation of microtubule associated proteins map2 and map u induces their functional alteration. Cell Structure & Function 15(6): 484