+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

The quantitative determination of Bacillus thuringiensis beta-exotoxin in insecticidal biopreparations

The quantitative determination of Bacillus thuringiensis beta-exotoxin in insecticidal biopreparations

Nauchnye Doklady Vysshei Shkoly. Biologicheskie Nauki 2: 119-126

ISSN/ISBN: 0470-4606

PMID: 2354222

A method of quantitative determination of beta-exotoxin content in liquid and dry bioformulations has been developed. The method includes a thin-layer chromatography to isolate beta-exotoxin from accompanying nucleotides, the further desorption of a single beta-exotoxin spot by water and to carry out spectrophotometry at 259 and 330 nm. beta-exotoxin content in industrial formulations bitoxibacillin and turingin I has been determined. The results obtained correspond to the NMR 1H spectroscopy data within the experimental errors. The relative error is 1-2%. The method sensitivity of 0.05 mg/ml. beta-exotoxin content at biotechnological stages of bitoxibacillin production has been determined.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 041728182

Download citation: RISBibTeXText

Related references

Quantitative determination of beta exotoxin of bacillus thuringiensis in insecticide biopreparations. Biologicheskie Nauki (Moscow) (2): 119-126, 1990

Quantitative analysis of beta-exotoxin in insecticidal preparations by high-performance anion-exchange chromatography Bacillus thuringiensis. Chemistry of natural compounds 18(3): 359-362, 1982

Isolement d'une β-endotoxine insecticide à partir de quelques sous-espèces de Bacillus thuringiensis - Isolation of an insecticidal beta-exotoxin from several subspecies of Bacillus thuringiensis. Res. Rep. Rural Dev. Adm 29(2): 66-72, 1987

Isolation of an insecticidal beta -exotoxin from several subspecies of Bacillus thuringiensis. Research Reports of the Rural Development Administration, Plant Environment, Mycology and Farm Products Utilization, Korea Re ic 29(2): 66-72, 1987

Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography. Journal of Bacteriology 172(6): 3172-3179, 1990

Determination of beta-exotoxin in Iranian isolates of Bacillus thuringiensis. Journal of Entomological Society of Iran 23(1): 55-68, 2003

The efficacy of the delta endotoxin of bacillus thuringiensis ssp tenebrionis strain bi 256 82 in comparison with the beta exotoxin of bacillus thuringiensis ssp thuringiensis strain i 5 against first instar larvae of the colorado potato beetle leptinotarsa decemlineata. Mededelingen van de Faculteit Landbouwwetenschappen Universiteit Gent 53(3 Part A): 1017-1022, 1988

A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner. Biochemical Journal 114(3): 477-488, 1969

High-performance liquid chromatographic determination of .beta.-exotoxin produced by the bacterium Bacillus thuringiensis. Journal of Agricultural and Food Chemistry 30(2): 407-408, 1982

Inhibition of de novo RNA synthesis by the insecticidal exotoxin of Bacillus thuringiensis var. gelechiae. Collection of Czechoslovak Chemical Communications 34(6): 1786-1791, 1969

The insecticidal action of the exotoxin of Bacillus thuringiensis on rodent flea larvae. Problemy Osobo Opasnykh Infektsii (1 (35)): 95-99, 1974

Larvicidal activity of the beta -exotoxin of Bacillus thuringiensis var. thuringiensis Berliner against two species of flies. Zeitschrift fur Angewandte Entomologie 74(2): 157-160, 1973

Sample preparation for beta-exotoxin determination in Bacillus thuringiensis cultures by reversed-phase high-performance liquid chromatography. Analytical Biochemistry 298(1): 112-117, 2001

Quantitative determination of the protein of insecticidal crystals of Bacillus thuringiensis by means of immuno-chemical methods. Biotekhnologiya 6(5): 89-92, 1990

Activity on the beta exotoxin of bacillus thuringiensis var thuringiensis in the escherichia coli dna repair assay for bacterial genotoxicity. Journal of Entomological Science 20(1): 104-108, 1985